Título

Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs

Autor

OSWALDO MARGARITO TORRES CHABLE

Ligia Guadalupe Brito Argáez

Ignacio Rodrigo Islas Flores

CLAUDIA VIRGINIA ZARAGOZA VERA

MARITZA ZARAGOZA VERA

GUADALUPE ARJONA JIMENEZ

CARLOS MARCIAL BAAK BAAK

NOHEMI CIGARROA TOLEDO

ROBERTO GONZALEZ GARDUÑO

CARLOS IGNACIO MACHAIN WILLIAMS

JULIAN EVERARDO GARCIA REJON

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/10.3855/jidc.12711

Materias

DIROFILARIA - (AUTORES) FILARIASIS - (AUTORES) IMMUNOREACTIVITY - (AUTORES) TROPICAL DISEASES - (AUTORES) ZOONOTIC - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOQUÍMICA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR - (CTI)

Resumen o descripción

Introduction: Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins. Methodology: Eight serum samples from the owners of microfilaremic dogs were examined. Total proteins were extracted from adult worms and 12% SDS-PAGE was performed. The gel was electroblotted to a nitrocellulose membrane, and a Western blot (WB) was performed. Reactive bands of 22, 33, 39, 49, and 63 kDa in WB were excised from the gel and analyzed by mass spectrometry (MS). Results: The MS results showed the presence of 10 different proteins of D. immitis recognized by the human serum samples. Conclusions: These results indicate that in endemic areas of D. immitis, owners of infected dogs recognize specific proteins of the parasite, suggesting a possible infection.

Fecha de publicación

2020

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1865

Formato

application/pdf

Fuente

J Infect Dev Ctries. 2020 Dec 31;14(12):1442-1447. doi: 10.3855/jidc.12711. PMID: 33378288.

Idioma

Inglés

Sugerencia de citación

Torres-Chable OM, Brito-Argaez LG, Islas-Flores IR, Zaragoza-Vera CV, Zaragoza-Vera M, Arjona-Jimenez G, Baak-Baak CM, Cigarroa-Toledo N, Gonzalez-Garduño R, Machain-Williams CI, Garcia-Rejon JE. Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs. J Infect Dev Ctries. 2020 Dec 31;14(12):1442-1447. doi: 10.3855/jidc.12711. PMID: 33378288.

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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