Título

Gene expression in primary hemocyte culture of the Pacific white shrimp Penaeus vannamei infected with different white spot syndrome virus (WSSV) strains

Autor

Delia Patricia Parrilla Taylor

REGINA ELIZONDO GONZALEZ

Jesús Neftalí Gutiérrez Rivera

SILVIA ALEJANDRA GARCIA GASCA

NORBERTO VIBANCO PEREZ

MA. DE JESUS DURAN AVELAR

RICARDO VAZQUEZ JUAREZ

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de publicación

doi: DOI: 10.3856/vol50-issue4-fulltext-2816

ISSN/ISSN: 0718-560X

URL/URL: https://www.lajar.cl/index.php/rlajar/article/view/vol50-issue4-fulltext-2816

Materias

Penaeus vannamei, virulence, gene expression, viral fitness, viral pathogenesis, immune response - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA ANIMAL (ZOOLOGÍA) - (CTI) PATOLOGÍA ANIMAL - (CTI) PATOLOGÍA ANIMAL - (CTI)

Resumen o descripción

"Five previously analyzed white spot syndrome virus (WSSV) strains from northwest Mexico,differing in their genome architecture as well as in virulence, were selected (high virulence JP and LG strains;moderate virulence GVE and DIV strains; and low virulence LC10 strain) to evaluate pathogenesis response in vitro. Expression of phagocytosis-activating protein PAP, manganese superoxide dismutase MnSOD and peroxiredoxin PRX, and two genes of immediate-early expression (IE1 and WSSV304) were measured by qPCR in a primary hemocyte cell culture from Penaeus vannamei at 1, 3, 6, 12, and 24 h post-infection (hpi). PAP expression was significantly higher at 1 and 3 hpi, and JP and LC10 strains induced the highest expression. The response of MnSOD was high at 1 hpi, and a significant increase in PRX expression was detected at 3 hpi, probably due to the occurrence of an oxidative burst; expression levels of MnSOD and PRX were significantly higher at 1 and 3 hpi, respectively, induced by the LG strain (high virulence), suggesting an acute response. In general, expression of most immune-related - genes decreased after the initial hours of infection. Expression levels of IE1 and WSSV304 were exceptionally high at 1 hpi in almost all five WSSV analyzed strains,confirming their efficient mechanism for replication and viral fitness. The results of this study do not show an accurate link between the genome size and WSSV virulence of the strains, albeit the strain with the smallest genome showed the highest virulence. All strains induced an early immune response in heterogeneous ways."

Editor

Latin American Journal of Aquatic Research

Fecha de publicación

2022

Tipo de publicación

Artículo

Recurso de información

http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/2834

Formato

application/pdf

Fuente

Latin American Journal of Aquatic Research

Idioma

Inglés

Sugerencia de citación

Parrilla-Taylor D, Elizondo-González R, Gutierrez-Rivera J, García-Gasca A, Vibanco-Pérez N, Durán Avelar M, Vázquez-Juárez R. Gene expression in primary hemocyte culture of the Pacific white shrimp Penaeus vannamei infected with different white spot syndrome virus (WSSV) strains. Lat. Am. J. Aquat. Res.. 2022;50(4): 562-572. Available from: doi:10.3856/vol50-issue4-fulltext-2816

Repositorio Orígen

Repositorio Institucional CIBNOR

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