Título

Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

Autor

ELIANA VALENCIA LOZANO

LISSET HERRERA ISIDRON

Osiel Salvador Recoder-Meléndez

Aarón Barraza Celis

JOSE LUIS CABRERA PONCE

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de publicación

doi: DOI: 10.3390/ijms232213835

URL/URL: https://www.mdpi.com/1422-0067/23/22/13835

ISSN/ISSN: 1422-0067

Materias

transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) GENÉTICA - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI)

Resumen o descripción

"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."

Editor

Multidisciplinary Digital Publishing Institute

Fecha de publicación

2022

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/2822

Formato

application/pdf

Fuente

International Journal of Molecular Sciences

Idioma

Inglés

Sugerencia de citación

Valencia-Lozano, E.; Herrera-Isidrón, L.; Flores-López, J.A.; Recoder-Meléndez, O.S.; Barraza, A.; Cabrera-Ponce, J.L. Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis. Int. J. Mol. Sci. 2022, 23, 13835. https:// doi.org/10.3390/ijms232213835

Repositorio Orígen

Repositorio Institucional CIBNOR

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