Título

Effect of glucoamylase enzyme extract on in vitro gas production and degradability of two diets with 25% of corn or sorghum grains

Autor

Abdelfattah Zeidan Mohamed Salem

Ahmed E. Kholif

MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

Corn Glucoamylase In vitro fermentation Sorghum CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

The optimal use of starch is fundamental in improving performance of ruminants fed high grain diets (Huntington 1997, Rojo et al. 2000). Many strategies were developed to increase starch digestion rate and grain energetic value such as ground, dry rolled and steamed, and harvest of grains with high moisture content (Owens et al. 1997). Amylolytic enzymes in the rumen are extracellular or cell-bound (Thurn and Kotarsky 1987), and the extracellular enzymes are the most important in the group of amylolytic bacteria (Cotta 1988). Amylases are present in protozoa (Mendoza et al. 1993 1995) and ruminal fungi (Yanke et al. 1993). Exogenous amylolytic enzymes are obtained from controlled fermentation of bacteria or fungi (Declerk et al. 1997) and they are used in the food industry for starch hydrolysis (Reilly 1985).

The objective of this study was to evaluate the effect of glucoamylase enzyme (GEZ) extract on the in vitro ruminal gas production (GP) and degradability of 2 total mixed rations (TMR) of 25% of corn and other of 25% of sorghum grains. The 2 diets were treated with 0, 1.5 and 3 g of GEZ protein (65% of protein) per kg of grain in diet. Diets GP were measured at 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of incubation. Incubations were stopped after 72 h where pH was measured and supernatant was filtered to determine in vitro dry matter (DMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) degradabilities. Addition of GEZ to corn diet had no effect on kinetics of GP, whereas GEZ added to sorghum diet, at the high dose of the enzyme (3 g/kg DM), was traduced by an increase of the rhythm of GP (c) and the volume of GP at 2, 4 and 6 h of incubation. Likewise, effect of GEZ was not affected either on the DMD or cell wall (NDFD and ADFD) of both diets (sorghum or corn). Irrespective to enzyme supply, kinetics of GP and pattern of degradation of corn were generally higher than those of sorghum. A net effect of the diet and the interactions between diet and enzyme were recorded for the volume of GP at different incubation times. The use of high doses of GEZ should be tested on the pattern rumen fermentation.

Editor

Indian Journal of Animal Sciences

Fecha de publicación

2 de octubre de 2014

Tipo de publicación

Artículo

Recurso de información

http://hdl.handle.net/20.500.11799/66035

Idioma

Inglés

Relación

Vol.;2

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DE LA UAEM

Descargas

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