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RUTH ESCAMILLA MONTES GENARO DIARTE PLATA GABRIELA BERENICE MENDOZA MALDONADO Aarón Barraza Celis Carlos Eliud Angulo Valadez Seyed Hossein Hoseinifar Jesús Arturo Fierro Coronado ANTONIO LUNA GONZALEZ (2022)
"Invertebrates' immune defense mechanisms play a critical role in pathogen recognition and elimination. De novo assembly and annotation of the Argopecten ventricosus transcriptome were performed for the immune-related gene identification. Scallops (height: 4.4 cm) were challenged with inactivated Vibrio parahaemolyticus IPNGS16. The RNA from different tissues was pooled for a single cDNA library construction sequenced by NextSeq 500 platform 2×75 paired‐end chemistry. Before de novo assembling with Trinity, reads were analyzed with FastQC, Trimmomatic, and Prinseq. Assembled sequences were analyzed by CD-HIT-EST and TransDecoder. The corresponding annotation was performed against NCBI-nr, RefSeq protein, and KAAS (KEGG) databases. The Trinity assembly yielded 107,516 contigs. TransDecoder yielded 25,285 sequences as CDSs of which, 16,123 were annotated against the NCBI-nr protein, most of them scored with Crassostrea gigas data. Gene ontology mapped sequences (15,262) were classified in molecular functions (~13,000), cellular components (~11,000), and biological processes (~13,000). The KAAS analysis showed biological categories for metabolism (13%), cellular processes (12%), genetic information processing (10%), organismal systems (19%), environmental information (13%), and human diseases (33 %). Within the organismal systems, 467 immune-related genes (KO) were identified. Sixty-four immune-related genes were annotated/blasted against the NCBI-nr and RefSeq protein databases. An RT-qPCR was performed to analyze the expression level of immune-related genes obtained in the transcriptome analysis in scallops (height 4.5 cm) treated with probiotic bacilli added to culture water. Bacilli significantly increased the expression of the HSP70 and PGRP genes. The gene transcripts analysis of A. ventricosus will better understand its immune response against pathogens in culture systems."
Article
Argopecten ventricosus, calico scallop, transcriptome, bivalve, immune genes BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) INMUNOLOGÍA INMUNOLOGÍA
Rice–wheat comparative genomics: Gains and gaps
Akila Wijerathna-Yapa Md. Harun-Or-Rashid BHOJA BASNET (2023)
Article
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA COMPARATIVE GENOMICS GENES GENETIC ENGINEERING BREEDING RICE WHEAT
Spurthi Nayak Polavarapu Kavi Kishor Rajeev Varshney (2010)
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Simple Sequence Repeats Mapping Population Translational Studies CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ANCHORS CHICKPEAS CICER ARIETINUM GENETIC MAPS GENETIC MARKERS MEDICAGO MEDICAGO TRUNCATULA MICROSATELLITES SINGLE NUCLEOTIDE POLYMORPHISM GENES
RNAseq of diverse spring wheat cultivars released during last 110 years
awais rasheed Huihui Li (2023)
Article
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA RNA SEQUENCE SPRING WHEAT ROOT ARCHITECTURE GENES
Harbans Bariana Lakshmi Kant Naeela Qureshi Urmil Bansal (2022)
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Kompetitive Allele Specific PCR Stripe Rust Yr Genes CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENETIC MARKERS MARKER-ASSISTED SELECTION RUSTS WHEAT GENES
Shailendra Sharma Avinash Kumar Apekshita Singh deepmala sehgal Shailendra Goel SoomNath Raina (2022)
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Immune Evasion Mutation Analysis Novel Spike Mutations Reduced Neutralization SARS-CoV-2 CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA COVID-19 NEUTRALIZATION SPIKES
Gopalareddy Krishnappa Govindan Velu (2023)
Article
DArT-Seq Gene Mapping Yield Component Traits CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT QUANTITATIVE TRAIT LOCI CANDIDATE GENES QUANTITATIVE TRAIT LOCI MAPPING YIELD COMPONENTS BIOFORTIFICATION
deepmala sehgal Laura Dixon Diego Pequeno Jose Crossa Alison Bentley Susanne Dreisigacker (2024)
Book part
Hexaploid Wheat Adaptive Genes Novel Genomic Regions Gene-Based Modeling Process-Based Modeling Global Food Security CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HEXAPLOIDY WHEAT QUANTITATIVE TRAIT LOCI MODELLING FOOD SECURITY
CAIXIA LAN Ravi Singh JULIO HUERTA_ESPINO Zaifeng Li Evans Lagudah sridhar bhavani (2022)
Article
Genetic Analysis Molecular Mapping Wheat Rusts APR Genes CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ADULT PLANT RESISTANCE LANDRACES WHEAT RUSTS