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Background: A common problem during pregnancy is anemia and to reduce its prevalence the WHO and national guidelines recommend a prescription of 30 to 60 mg of iron/day. The aim of this study was to evaluate the association of iron profile, hepcidin and oxidative stress in pregnant women prescribed with iron as a probable cause of metabolic disorders. Method: In this cohort study two groups were followed: A) women with low-risk pregnancy (WLRP), B) women with high-risk pregnancy (WHRP): women with metabolic disorders (dyslipidemias, GDM and high blood pressure). Oxidative stress enzymes, iron profile and hepcidin were measured in the second and third trimesters. Results: There were significant differences in hepcidin levels between WLRP and WHRP in 2nd (3.6 ± 4.2 vs 4.69 ± 3.23 P=0.005) and 3rd trimester (3.65 ± 3.44 vs 6.84 ± 5.14 P=0.02). The serum iron concentration had a negative relationship with catalase (-0.599; P=0.04) and a positive relationship with glutathione peroxidase (0.729; P=0.007). Conclusion: The iron serum levels increase could induce oxidative damage in pregnancy. Increased hepcidin is a useful biomarker for determining iron availability in pregnancy and its association with antioxidant systems.
Govindan Velu (2022)
Forty-eight growing New Zealand White male rabbits aged 6 weeks (874±1.3 g initial body weight (BW)) were used to study effects of partial replacement of berseem hay (BH) with Salix tetrasperma hay (ST) on growth and physiological responses. Rabbits were allotted to one of four diets of 12 rabbits each for 75 days in a completely randomized design. The treatments were as follows: control (30 % BH), ST25 (7.5 % ST+22.5 % BH), ST50 (15 % ST+15 % BH), ST75 (22.5 % ST+7.5 % BH). Nutrient digestibility coefficients, nutritive value and N utilization of rabbits fed with the ST50 rations were higher (P<0.05) than the other groups. Final live BW, average daily gain, feed intake and feed efficiency of rabbits fed ST25 and ST50 were higher (P<0.05) than those fed ST75 and the control. Serum biochemical metabolites of urea, creatinine, aspartate amino transferase and alanine amino transferase concentrations varied among diets, with the rank order (P<0.05) ST75>ST25 and ST50>control. Glucose level was higher (P<0.05) for the control than the other diets. Rabbits fed with the mixed diets of ST had lower (P<0.05) total lipids, cholesterol and triglycerides levels than those fed with control. Haematological indices of packed cell volume, haemoglobin, red blood cells, white blood cells and lymphocyte counts were lower (P<0.05), but monocyte was higher, in rabbits fed with the ST75 than the other groups. However, other haematological parameters were similar among diets. Since all the performance and blood parameters were within normal ranges for healthy rabbits, and there were no signs of toxicity, we conclude that partial replacement of BH by ST improves rabbit growth performance, and did not impact rabbit health.
Oxidative stress (OS) is considered a risk factor for neurodegenerative disorders. Iron concentrations have been related to OS; however, the effect of iron deficiency (ID) on the induction of OS in the central nervous system (CNS) is unknown. A murine model of chronic diet-induced ID using 2 month-old male BALB/c mice (6 specimen), was used to determine its role in OS induction. Lipid peroxidation was determined by measuring thiobarbituric acid-reactive species (TBARS) in the CNS, using the spleen for comparison. A decrease of peroxidation products in the CNS of the ID group (ID CNS), compared to controls fed on a regular diet, was found. The spleen showed a significant increase of lipid peroxidation in the ID group. Our results suggest that chronic ID may have differential effects on OS in the CNS and peripheral tissues. A decreased amount of reactive oxygen species in the basal state may be related to alterations on normal CNS metabolism and functions under chronic ID.
Multidisciplinarias (Ciencias Sociales) Oxidative stress lipid peroxidation iron deficiency central nervous system spleen Multidisciplinarias (Ciencias Sociales) Oxidative stress lipid peroxidation iron deficiency central nervous system spleen CIENCIAS SOCIALES
Multiwalled Carbon Nanotubes (MWCNTs) were used as a support of amperometric enzymatic biosensors of guaiacol. The structural quality of MWCNTs was determined for Raman Spectra and DRX analysis. MWCNTs were decorated with iron oxide nanoparticles (36 w/w%), which were observed by FE-SEM, and were confirmed by with EDX, and TGA analysis. Laccase enzyme (aspergillus sp.) was immobilized on the surface of MWCNTs (oxidized and decorated with iron oxide nanoparticles) confirmed by XPS analysis and used to amperometric detection of guaiacol. The material obtained was deposited on the active surface of glassy carbon electrode (GCE) and was carried out using a typical three-electrode system with saturated calomel electrode as a reference and a graphite rod as a counter-electrode. The results confirm the potential use of bioelectrode Lac/MWCNTs/GCE and Lac/Fe3O4/MWCNTs/GCE for the guaiacol detection in low concentrations. Amperometric sensitivities and detection limits of Lac/Fe3O4/MWCNTs/GCE bioelectrode (110.186 μA mMcm−2 and 34.301 nM for reduction current respectively) showed better results than Lac/MWCNTs/GCE bioelectrode in a linear range 0–0.066 μM of guaiacol.
ALCOHOLS BIOSENSORS ENZYMES GLASS MEMBRANE ELECTRODES IRON METALLOGRAPHY IRON OXIDES MAGNETIC NANOPARTICLES METAL NANOPARTICLES NANOTUBES INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA ENERGÉTICA ENERGÍA ENERGÍA
"DNA extraction and purification is considered a critical step in different biomedical applications such as genetic therapy and clinical diagnosis. This research describes the synthesis and characterization of functionalized IONPs with potential applications in plasmid DNA isolation. IONPs were synthesized by the chemical coprecipitation method followed by a post-synthesis functionalization using silica and (3-aminopropyl)triethoxysilane (APTES). A second functionalization strategy was carried out by an in situ coprecipitation of Fe(II) and Fe(III) ions in presence of chitosan and tris(hydroxymethyl)aminomethane (Tris). IONPs characterization by X-Ray diffraction (XRD) confirmed the synthesis of inverse-spinel magnetite like nanoparticles. In addition, infrared spectroscopy allowed to identify the hydroxyl, silanol and amino functional groups on the surface of the nanoparticles. Transmission electron microscopy measurements revealed IONPs with an average particle size under 13 nm. According to saturation and remanence magnetization values, all samples were suitable for bioseparation studies using magnetic manipulation. Preliminary separation assays with oligodeoxynucleotides (ODN) and plasmid DNA (pDNA) were carried out. Furthermore, biomolecular integrity of ODN and pDNA was verified using polyacrylamide and agarose gel electrophoresis, respectively. Synthesized IONPs and specially those functionalized with silica, chitosan and Tris presented comparable desorption percentages with some reported studies in plasmid DNA separation. Remarkably, by using such functionalized IONPs, the DNA desorption times were more than ten-time faster than other similar reported adsorbents. Therefore, they can be considered for DNA extraction and purification from complex biological samples."
El hierro dúctil (DI) puede modificar sus propiedades con la adición de elementos aleantes y tratamientos térmicos adecuados. En este trabajo se estudiaron los efectos del vanadio y molibdeno en la microestructura y resistencia a la corrosión del DI y el hierro dúctil austemperizado (ADI). La resistencia a la corrosión fue evaluada por la técnica de polarización potencio-dinámica en 0.5 M H2SO4, 0.5 M NaCl y 0.5 M NaOH como electrólito. El ADI aleado con vanadio presenta ausferrita gruesa formada por agujas gruesas de ferrita, mientras que la combinación de vanadio y molibdeno permite obtener una microestructura fina compuesta de ausferrita y agujas delgadas de ferrita; esta microestructura mejora la resistencia a la corrosión en NaCl y NaOH. El DI presenta corrosión debido al par galvánico entre los nódulos de grafito y la ferrita; sin embargo, la presencia de una gran cantidad de carburos incrementa su resistencia a la corrosión en H2SO4.
Ductile iron (DI) can acquire various properties with the addition of alloying elements and through heat treatment. In this work, the effects of vanadium and molybdenum on the microstructure and corrosion resistance of DI and austempered ductile iron (ADI) were studied. Corrosion resistance was evaluated by potentiodynamic polarization techniques in 0.5 M H2SO4, 0.5 M NaCl, and 05 M NaOH as electrolyte. The ADI alloyed with vanadium presented an upper ausferritic microstructure consisting of broad ferrite needles, while the combination of vanadium and molybdenum allows obtaining a fine microstructure composed of ausferrite and thin needles of ferrite; this microstructure improves the corrosion resistance in NaCI and NaOH. The DI’s showed corrosion due to the galvanic pair between the graphite nodule and the ferrite; however, a high amount of carbide increases the corrosion resistance in H2SO4.
INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA METALÚRGICA ADI (Austempered Ductile Iron) Vanadium Molybdenum Microstructure Corrosion ADI (Hierro Dúctil Austemperizado) Vanadio Molibdeno Corrosión