Advanced search


Knowledge area




Filter by:

Publication type

Authors

Issue Years

Publishers

Origin repository

Access Level

Language

Subject

Select the topics of your interest and receive the hottest publications in your email

11 results, page 1 of 2

Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy

CECILIA GUIZAR GONZALEZ MIRIAM DEL SOCORRO MONFORTE GONZALEZ FELIPE AUGUSTO VAZQUEZ FLOTA (2016)

Exposure to YE promoted sanguinarine accumulation. This was not observed when they were exposed to methyl jasmonate (MeJa). Use of diethyldithiocarbamic acid (DIECA), an inhibitor of the OCDA pathway, resulted in partial impairment of this response. Exogenous application of MeJa did not reverse this effect in DIECA-exposed cultures. qRT-PCR revealed that the accumulation of transcripts corresponding to the berberine bridge enzyme gene, which was induced by YE exposure, was blocked by OCDA pathway and reversed by exogenous MeJa. Interestingly, this response pattern could not be observed on dihydrobenzophenanthridine oxidase enzyme activity, which was promoted by YE, but unaffected by either OCDA or MeJa.

Article

ARGEMONE MEXICANA BENZOPHENANTHRIDINES OCDA PATHWAY SANGUINARINE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA

Transcritos de genes de defensa acumulados en chile CM334 infectado por Nacobbus aberrans o por Meloidogyne enterolobii e inoculado con Phytophthora capsici

EDGAR VILLAR LUNA (2013)

Tesis (Doctorado en Ciencias, especialista en Fitopatología).- Colegio de Postgraduados, 2013.

El chile CM334 es resistente al oomiceto Phytophthora capsici (Pc) y a los nematodos Meloidogyne incognita, M. arenaria y M. javanica, pero susceptible a M. enterolobii (Me) y Nacobbus aberrans (Na). La resistencia de Capsicum annuum a Pc se asocia con la acumulación de la fitoalexina sesquiterpenica capsidiol y con la sobre-expresión de varios genes de defensa. Los objetivos de este trabajo fueron: 1) conocer las modificaciones que Na y Me inducen en la expresión de algunos genes de la ruta mevalónica (HMG2, EAS, HMG3, y SS) en plantas CM334 infectadas por Na (NaPc) o Me (MPc) e inoculadas con Pc (Pc); 2) determinar la relación entre los niveles de HMG2 y EAS en raíces de CM334 inoculadas sólo con Na (N), con Pc (Pc) y con ambos patógenos (NPc); y 3) evaluar los niveles de transcritos de WRKY-a, WRKY1, POX, PR-1 y EAS en agallas de raíces infectadas por Na y compararlos con los acumulados en las inoculadas sólo con Pc. En tallos de plantas de los tratamientos NaPc y MPc, los niveles de HMG2, HMG3, EAS, y SS fueron significativamente (P≤0.05) inferiores al testigo Pc, cuando el oomiceto se inoculó a los 3 y 21 días posteriores a la inoculación (dpi) con Na o Me; en NaPc y MPc los niveles de capsidiol en tallos fueron inferiores (P≤0.05) únicamente cuando Pc se inoculó a los 21 dpi con el nematodo, en comparación con el tratamiento Pc; en cambio, en raíces las diferencias entre Pc vs NaPc y Pc vs MPc fueron significativas (P≤0.05) tanto a 3 como a 21 dpi. En raíces, los niveles de HMG2 y EAS fueron reducidos (P≤0.05) en el tratamiento N en comparación con Pc, mientras que los de NPc fueron superiores a los de N, pero generalmente inferiores a los de Pc; ambos genes estuvieron positivamente correlacionados. Los niveles de WRKY1, POX, y PR-1 fueron inferiores (P≤0.05) en el tratamiento Na en comparación con Pc; y los de WRKY-a y EAS fueron reprimidos localmente en presencia del nematodo; en cambio en Pc, todos los genes fueron significativamente (P≤0.05) sobre-expresados. Na y Me modificaron los patrones de expresión de genes de defensa y los niveles de capsidiol. _______________ DEFENSE GENE TRANSCRIPTS ACCUMULATION IN CHILI PEPPER CM334 INFECTED BY Naccobus aberrans OR Meloidogyne enterolobii AND INOCULATED WITH Phytophthora capsici. ABSTRACT: Chili pepper CM334 is resistant to the oomycete Phytophthora capsici (Pc) and the nematodes Meloidogyne incognita, M. arenaria and M. javanica, but susceptible to M. enterolobii (Me) and Nacobbus aberrans (Na). Capsicum annuum resistance to Pc is associated with the accumulation of sesquiterpene phytoalexin capsidiol and overexpression of several defense-related genes. The objectives of this study were: 1) to know what changes in the expression of some mevalonate pathway genes (HMG2, EAS, HMG3, y SS) occur in CM334 plants infected by Na (NaPc) or Me (MPc) and inoculated with Pc (Pc); 2) to determine the relationship between levels of HMG2 and EAS in CM334 roots inoculated only with Na (N), with Pc (Pc) and with both pathogens (NPc); and 3) to assess the levels of WRKY-a, WRKY1, POX, PR-1 and EAS transcripts in infected root galls and compare them with those accumulated in roots inoculated only with Pc. In stems of NaPc and MPc treatments, HMG2, HMG3, EAS, and SS levels were significantly (P≤0.05) lower than in the Pc treatment, when the oomycete was inoculated at 3 and 21 days after inoculation (dai) with Na or Me; in NaPc and MPc treatments, capsidiol levels in stems were lower (P≤0.05) only when Pc was inoculated at 21 dai with nematode, compared with Pc treatment, whereas in roots differences between Pc vs NaPc and Pc vs MPc were significant (P≤0.05) at 3 and 21 dai. In roots, HMG2 and EAS transcript levels were reduced (P≤0.05) in N treatment compared to Pc, while those of NPc were higher to those of N, but generally lower than Pc, both genes were positively correlated. The WRKY1, POX, and PR-1 transcript levels were lower (P≤0.05) in Na treatment compared to Pc; WRKY-a and EAS genes were locally repressed in the presence of nematode, whereas in Pc all genes were significantly overexpressed (P≤0.05). Both nematodes (Na and Me) altered the pattern expression of defense genes and levels of capsidiol.

Doctoral thesis

Capsicum annuum Nematodos agalladores qRT-PCR Factores de transcripción Ruta mevalónica Root-knot nematodes Transcription factors Mevalonate pathway Fitopatología Doctorado CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Comparación de la expresión de los genes de defensa EAS, HMG2, WRKY-a, PR-1 y POX, y acumulación de capsidiol en la interacción compatible CM334-Nacobbus aberrans e incompatible CM334-Meloidogyne incognita

HERNAN VILLAR LUNA (2014)

Tesis (Maestría en Ciencias, especialista en Fitopatología).- Colegio de Postgraduados, 2014.

La línea de chile serrano CM334 es altamente resistente al oomiceto Phytophthora capsici y a las tres principales especies de nematodos agalladores (Meloidogyne arenaria, M. incognita y M. javanica), pero susceptible a M. enterolobii y Nacobbus aberrans. Su resistencia a Meloidogyne spp se asocia con la sobre-expresión de genes que codifican para los factores de transcripción WRKY2 y WRKY-a y con la acumulación de ácido clorogénico, y en el oomiceto con la sobre-expresión de varios genes de defensa y una elevada acumulación de la fitoalexina capsidiol. El objetivo de este trabajo fue estimar mediante qPCR los niveles de expresión de los genes de defensa EAS, HMG2, WRKY-a, PR-1 y POX, en la interacción compatible CM334/N. aberrans y en la incompatible CM334/M. incognita; y comparar los niveles de acumulación de la fitoalexina capsidiol en ambas interacciones. Todos los genes de defensa se sobre-expresaron en las dos interacciones, pero, tanto la sobre-expresión como la acumulación de capsidiol siempre fue mayor en las plantas inoculadas con M. incognita, en comparación con la interacción compatible CM334/N. aberrans; aunque las diferencias en la sobreexpresión no siempre fueron significativas (P= 0.05). Los mayores incrementos inducidos por M. incognita, se relacionaron con la restricción en su establecimiento y reproducción, como lo indica el hecho de que solamente el 0.1% de los juveniles se encontrara dentro de la raíz a los 21 días después de haber realizado la inoculación. _______________ COMPARISON OF EXPRESSION OF DEFENSE GENES EAS, HMG2, WRKY-a, PR-1 AND POX, AND ACCUMULATION OF CAPSIDIOL IN THE COMPATIBLE INTERACTION CM334-Nacobbus aberrans AND INCOMPATIBLE CM334-Meloidogyne incognita. ABSTRACT: The line chili pepper CM334 is highly resistant to the oomycete Phytophthora capsici and the three major species of root-knot nematodes (Meloidogyne arenaria, M. incognita and M. javanica), but susceptible to M. enterolobii and Nacobbus aberrans. Resistance to Meloidogyne spp is associated with the overexpression of genes encoding the transcription factors WRKY and WRKY2-a and the accumulation of chlorogenic acid, and in the oomycete with the overexpression of several genes of defense and high accumulation of the phytoalexin capsidiol. The objective of this study was to estimate by qPCR the expression levels of the defense genes EAS, HMG2, WRKY-a, PR-1 and POX in the compatible interaction CM334 / N. aberrans and the incompatible CM334 / M. incognita; and to compare the accumulation of capsidiol in both interactions. All defense genes were overexpressed in the two interactions, but in both overexpression and the accumulation of capsidiol was always higher in plants inoculated with M. incognita compared to the compatible interaction CM334 / N. aberrans; although overexpression differences were no always significant (P=0.05). The highest increases induced by M. incognita were associated with the restriction in its establishment and reproduction, indicated by the fact that only 0.1% of the juveniles were found within the root at 21 days after inoculation.

Master thesis

Capsicum annuum qPCR Factores de transcripción Nematodos agalladores Ruta mevalónica Transcription factor WRKY-a Root-knot nematodes Mevalonic pathway Fitopatología Maestría CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Filtros de lealtad y elección curricular en la profesión del turismo

Loyalty filters and curriculum election in the tourism profession

EVANGELINA CRUZ BARBA (2019)

El objetivo de este trabajo es presentar un modelo teórico de economía de la educación con base en la teoría de juegos. Se expone el caso de la Licenciatura en Turismo de la Universidad de Guadalajara al identificarse dos opciones académicas: la ruta académica S, considerada como tradicional, ola ruta P, considerada como alternativa. La idea central es que el estudiante utiliza sus recursos personales con sesgos en la racionalidad durante el proceso de elección académica dentro de un plan de estudios que opera bajo un sistema educativo por créditos. En el modelo se considera la asimetría de la información, los saberes y las experiencias previas de los estudiantes, que revelan el proceso cognitivo implicado en la elección académica. Sin embargo, el estudiante tiene filtros de lealtad que incidenen su elección. Por tanto, se presenta un problema de agencia que se resuelve mediante el señalamiento. Se concluye que la elección dependerá del proceso cognición-maximización basado en la información disponible y filtros de lealtad del estudiante, asumiendo que la manifestación de los sesgos en la elección puede provocar una ruptura entre elección curricular y mercado labora

A game theory model is presented under the agency-principal approach in order to analyze the process of curriculum choice under the credit system schedule in the BA in tourism at the University of Guadalajara. Considering asymmetric information, the previous knowledge and the labor experiences, the students choose their academic pathway (academic option) in order to maximize their benefits. They have two options: option S, the traditional one; and option P, the alternative. However, the students have loyalty filters affecting their decision. The solution to this game is given by the signaling. The students choose the option with the best reputation considering the available information, their loyalty filters, and the way in which the cognitive maximization is given.It is assumed that the manifestation of the biases in the election may originate a rupture between the curricular choice and the labor market

Article

HUMANIDADES Y CIENCIAS DE LA CONDUCTA Economía de la educación Elecciones Proceso cognitivo Educación superior Turismo Economics of education Agency theory Professional election Academic pathway Tourism

Circulating levels of specific members of chromosome 19 microRNA cluster are associated with preeclampsia development

MARGARITA DE LA LUZ MARTINEZ FIERRO IDALIA GARZA VELOZ JOSE MANUEL ORTIZ RODRIGUEZ RODRIGO CASTAÑEDA MIRANDA LUIS OCTAVIO SOLIS SANCHEZ (2017)

Purpose: To perform serum microRNA expression profiling to identify members of chromosome 19 miRNA cluster involved in preeclampsia development.

Methods: Serum chromosome 19 miRNA cluster microRNA expression profiling was evaluated at 12, 16, and 20 gestational weeks and at the time of preeclampsia diagnosis, in women who developed preeclampsia (WWD-PE; n = 16) and controls (n = 18) using TaqMan low density array plates.

Results: A total of 51 chromosome 19 microRNA cluster members were evaluated. The circulating hsa-miRs 512-3p, 518f3p, 520c-3p, and 520d-3p, were differentially expressed between groups (P < 0.05). Compared with controls, serum levels of hsa-miR-518f-3p at 20 GW were useful for identifying WWD-Mild-PE (P = 0.035) and WWD-Severe-PE(P = 0.007).

Conclusions: Serum hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, are differentially expressed between WWD-PE and controls and their role in the development of preeclampsia should be investigated further.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

INGENIERÍA Y TECNOLOGÍA Biomarkers Preeclampsia  Placenta Signaling pathway MicroRNA

Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

EMILIO MEDINA RIVERO VICTOR EMMANUEL BALDERAS HERNANDEZ LEANDRO GABRIEL ORDONEZ ACEVEDO LUZ MARIA TERESITA PAZ MALDONADO ANA PAULINA BARBA DE LA ROSA ANTONIO DE LEON RODRIGUEZ (2007)

"Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction."

Article

Periplasm Signal peptide Tat pathway Therapeutic protein CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Identifying high-yield low-emission pathways for the cereal production in South Asia

Tek Sapkota Jeetendra Aryal Arun Khatri-Chhetri Paresh Shirsath Clare Stirling (2018)

Increasing agricultural production to meet the growing demand for food whilst reducing agricultural greenhouse gas (GHG) emissions is the major challenge under the changing climate. To develop long-term policies that address these challenges, strategies are needed to identify high-yield low-emission pathways for particular agricultural production systems. In this paper, we used bio-physical and socio-economic models to analyze the impact of different management practices on crop yield and emissions in two contrasting agricultural production systems of the Indo-Gangetic Plain (IGP) of India. The result revealed the importance of considering both management and socio-economic factors in the development of high-yield low-emission pathways for cereal production systems. Nitrogen use rate and frequency of application, tillage and residue management and manure application significantly affected GHG emissions from the cereal systems. In addition, various socio-economic factors such as gender, level of education, training on climate change adaptation and mitigation and access to information significantly influenced the adoption of technologies contributing to high-yield low-emission pathways. We discussed the policy implications of these findings in the context of food security and climate change.

Article

Cereals Production Cereal Systems High-Yield Low Emission Pathway GREENHOUSE GAS EMISSIONS CLIMATE CHANGE CEREAL CROPS CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Apoptosis activation in human lung cancer cell lines by a novel synthetic peptide derived from Conus californicus venom

IRASEMA OROZ PARRA (2016)

"Lung cancer is one of the most common types of cancer in men and women and a leading cause of death worldwide resulting in more than one million deaths per year. The venom of marine snails Conus contains up to 200 pharmacologically active compounds that target several receptors in the cell membrane. Due to their diversity and specific binding properties, Conus toxins hold great potential as source of new drugs against cancer. We analyzed the cytotoxic effect of a 17-amino acid synthetic peptide (s-cal14.1a) that is based on a native toxin (cal14.1a) isolated from the sea snail Conus californicus. Cytotoxicity studies in four lung cancer cell lines were complemented with measurement of gene expression of apoptosis-related proteins Bcl-2, BAX and the pro-survival proteins NFκB-1 and COX-2, as well as quantification of caspase activity. Our results showed that H1299 and H1437 cell lines treated with s-call4.1a had decreased cell viability, activated caspases, and reduced expression of the pro-survival protein NFκB-1. To our knowledge, this is the first report describing activation of apoptosis in human lung cancer cell lines by s-cal14.1a and we offer insight into the possible mechanism of action."

Article

Lung cancer, Synthetic peptide, Apoptotic-related genes, Caspase-3 and -7, Apoptosis, Pathway BIOLOGÍA Y QUÍMICA

The Class II Trehalose 6-phosphate Synthase Gene PvTPS9 Modulates Trehalose Metabolism in Phaseolus vulgaris Nodules

Aarón Barraza Celis CECILIA CONTRERAS CUBAS GEORGINA ESTRADA NAVARRETE JOSE LUIS REYES TABOADA MARCO ADAN JUAREZ VERDAYES NELSON AVONCE VERGARA MA. DEL CARMEN MONSERRAT QUINTO HERNANDEZ CLAUDIA DIAZ CAMINO FEDERICO ESTEBAN SANCHEZ RODRIGUEZ (2016)

"Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamental role in plant growth and development, as well as in plant-microbe interactions. The expression of genes for trehalose synthesis during nodule development suggests that this metabolite may play a role in legume-rhizobia symbiosis. In this work, PvTPS9, which encodes a Class II trehalose-6-phosphate synthase (TPS) of common bean (Phaseolus vulgaris), was silenced by RNA interference in transgenic nodules. The silencing of PvTPS9 in root nodules resulted in a reduction of 85% (± 1%) of its transcript, which correlated with a 30% decrease in trehalose contents of transgenic nodules and in untransformed leaves. Composite transgenic plants with PvTPS9 silenced in the roots showed no changes in nodule number and nitrogen fixation, but a severe reduction in plant biomass and altered transcript profiles of all Class II TPS genes. Our data suggest that PvTPS9 plays a key role in modulating trehalose metabolism in the symbiotic nodule and, therefore, in the whole plant."

Article

Class II trehalose-6-phosphate synthase, trehalose biosynthetic pathway, legume-rhizobiumsymbiosis signaling, AGO1-complex, Phaseolus vulgaris (common bean) BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) FISIOLOGÍA VEGETAL FISIOLOGÍA VEGETAL

Identificación y caracterización de las células germinales primordiales (CGPs) en embriones y larvas de Totoaba macdonaldi

Identification and characterization of primordial germ cells (PGCs) in embryos and larvae of Totoaba macdonaldi

RAQUEL ESCUREDO VIELBA (2018)

La totoaba (Totoaba macdonaldi), es un pez endémico del Golfo de California con alto potencial tanto económico como ecológico, sin embargo, en la actualidad, es una especie en peligro de extinción. Por lo tanto, el propósito de esta tesis fue caracterizar las células germinales primordiales (CGPs) de este pez perteneciente a la familia Sciaenidae, con dos objetivos: (1) proporcionar la base para la criopreservación de las CGPs para conservar el recurso genético y (2) dar el primer paso para conocer la génesis gonadal y la diferenciación sexual de este pez. El análisis de inmunofluorescencia realizado desde la etapa de 2 células hasta 8 días después de la eclosión (DDE) muestra que la proteína VASA es específica de las CGPs. Estas células se observaron por primera vez en las regiones periférica y dorsal del blastodisco, en aproximadamente el estadio del 50% -epibolia y migraron hacia ambos lados del cuerpo del embrión durante el desarrollo. Finalmente, a los 7 DDE, las CGPs del embrión alcanzaron la zona donde se desarrollará la gónada. El análisis histológico de las larvas mostró el primordio gonadal con un grupo de CGPs en el lado dorsal del peritoneo a los 11 DDE. El crecimiento de la gónada, como resultado de la interacción entre las CGPs y las células somáticas derivadas del peritoneo, se observó a los 16 DDE. Los resultados de qPCR mostraron que la expresión del gen Vasa estaba restringida al desarrollo embrionario y larval temprano, los valores más altos se observaron en la etapa de 2 células y de blástula media, lo que sugiere la herencia materna del ARNm de Vasa. Estos hallazgos apoyan la hipótesis de preformación en las CGPs de T. macdonaldi. El patrón de migración de las CGPs en esta especie, nos permite recomendar el aislamiento y la posterior criopreservación de estas células antes de los 7 DDE cuando el desarrollo embrionario y larval se da a 21 ° C.

The totoaba (Totoaba macdonaldi) is an endemic fish of the Gulf of California with high economic and ecological potential, however, at present, it is an endangered species. Therefore, our purpose was to characterize the Primordial Germ Cells (PGCs) of this Sciaenid with two objectives: (1) to provide the basis for PGCs cryopreservation to preserve the genetic resources and (2) to take the first step to know the gonadal genesis and sex differentiation of this fish. Immunofluorescence analysis performed from 2-cell stage to 8-day after hatch (DAH) shows that VASA protein is specific for PGCs. These cells were first observed in the peripheral and dorsal regions of the blastodisc at approximately the 50%-epiboly stage and migrated to both sides of embryo body during the development. Finally, at 7 DAH the PGCs of the hatching embryo reached the place where the gonad will develop. Histology analysis of larvae showed a genital ridge with enclosed PGCs on the dorsal side of the peritoneum at 11 DAH, gonadal primordium growth was observed as a result of the interaction between PGCs and somatic cells derived from the peritoneum. Results of qPCR showed that Vasa expression was restricted to the embryonic and early larval development, the highest values were observed in 2-cell and mid-blastula stage suggesting the maternal inheritance of Vasa mRNA. These findings support the hypothesis of preformation in T. macdonaldi PGCs. The migration pattern of PGCs allow us to recommend the isolation and subsequent cryopreservation of these cells before 7 DAH when temperature during embryonic and larval development is 21°C.

Doctoral thesis

Células germinales primordiales, Expresión Vasa, Proteína VASA, Ruta migratoria, Totoaba macdonaldi, Familia Scianidae, Primordial germ cells, Vasa expression, VASA protein, migration pathway, Totoaba macdonaldi, Scianidae family BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA CELULAR OTRAS OTRAS