Título
Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis.
Autor
Esperanza Duarte Escalante
Maria Guadalupe Frias De Leon
Luz Martínez
Jorge Herrera
Gustavo Acosta Altamirano
Carlos Cabello Gutierrez
Gabriel Palma
Maria del Rocio Alicia Reyes Montes
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: 10.3390/molecules23123145
Materias
Micología - ([Molecules (1420-3049 ) vol. 23(12), 1-15 (2018)]) Coccidioides spp - ([Molecules (1420-3049 ) vol. 23(12), 1-15 (2018)]) Coccidioidomicosis - ([Molecules (1420-3049 ) vol. 23(12), 1-15 (2018)]) Immunodiagnóstico - ([Molecules (1420-3049 ) vol. 23(12), 1-15 (2018)]) Péptidos - ([Molecules (1420-3049 ) vol. 23(12), 1-15 (2018)]) MEDICINA Y CIENCIAS DE LA SALUD - (CTI)
Resumen o descripción
Antigenic fractions of 100, 50, 37, and 28 kDa obtained through the SDS-PAGE method that were more frequently recognized by anti-Coccidioides antibodies in the sera of coccidioidomycosis patients were selected using western blotting. Subsequently, these bands were sequenced, and the obtained proteins were analysed by BLAST to choose peptides specific for Coccidioides spp. from among the shared aligned sequences of related fungi. A peptide specific for C. immitis was selected from the “GPI anchored serine-threonine rich protein OS C. immitis”, while from the “uncharacterized protein of C. immitis”, we selected a peptide for C. immitis and C. posadasii. These proteins arose from the 100 kDa antigenic fraction. From the protein “fatty acid amide hydrolase 1 of C. posadasii” that was identified from the 50 kDa antigenic fraction, a peptide was selected that recognized C. immitis and C. posadasii. In addition, the analysis of all the peptides (353) of each of the assembled proteins showed that only 35 had 100% identity with proteins of C. immitis and C. posadasii, one had 100% identity with only C. immitis, and one had 100% identity with only C. posadasii. These peptides can be used as diagnostic reagents, vaccines, and antifungals.
Editor
Multidisciplinary Digital Publishing Institute (MDPI)
Fecha de publicación
2018
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Molecules (1420-3049 ) vol. 23(12), 1-15 (2018)
Idioma
Inglés
Relación
https://www.mdpi.com/1420-3049/23/12
Cobertura
CH
Repositorio Orígen
Repositorio Institucional de la Facultad de Medicina,UNAM.Departamento de Microbiologia y Parasitologia
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