Título
First report of multiple anthelmintic resistance in goat farm in Cuba
Autor
Javier Arece García
Yoel López Leyva
AGUSTIN OLMEDO JUAREZ
GABRIEL RAMIREZ VARGAS
DAVID EMANUEL REYES GUERRERO
María Eugenia López Arellano
Pedro Mendoza de Gives
Michal Varady
ROLANDO ROJO RUBIO
ROBERTO GONZALEZ GARDUÑO
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
This study determined the efficacies of four classes of anthelmintics (AH) in a goat flock where anthelmintic resistance (AR) to ivermectin was suspected. We selected and randomly distributed 105 animals with >500 eggs per gram of feces into seven groups of 15 animals: an untreated con- trol group and groups treated with ivermectin, eprinomectin, albendazole sulfoxide, albendazole, levamisole, and closantel. The goats were individually weighed and treated with the recommended dose. Fecal samples were collected 14 days post-treatment to determine the fecal egg counts and for a fecal egg count reduction test (FECRT). Coprocultures were also performed for identifying any resistant genera. A molecular assay (polymerase chain reaction, PCR) was used to confirm benzimidazole resistance. The FECRTs for the ivermectin, eprinomectin, albendazole sulfoxide, and albendazole treatments were <90 %, indicating multiple anthelmintic resistance, all in Haemonchus spp. Levamisole had a FECRT confidence interval <90 %, indicating a moderate level of AR. The PCR detected the ß-tubulin alleles responsible for benzimidazole resistance, confirming AR. This study is the first monospecific report of AR in goats in Cuba, with a total failure of macrocyclic lac- tones anthelmintic class.
The project was funded by the Cuban Ministry of Agriculture (Pro- ject No. P131LH001-0072).
Editor
GRUYTER
Fecha de publicación
19 de julio de 2017
Tipo de publicación
Artículo
Recurso de información
Fuente
1336-9083
Idioma
Inglés
Audiencia
Estudiantes
Investigadores
Repositorio Orígen
REPOSITORIO INSTITUCIONAL DE LA UAEM
Descargas
180