Título

Glyphosate targets fish monoaminergic systems leading to oxidative stress and anxiety

Autor

Melissa Faria

Juliette Bedrossiantz

JONATHAN RICARDO ROSAS RAMIREZ

Marta Mayol

GERARDO HEREDIA GARCIA

Marina Bellot

Eva Prats

Natàlia Garcia-Reyero

Cristian Gómez-Canela

Leobardo Manuel Gómez Olivan

Demetrio Raldua

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Materias

Glyphosate - ([0160-4120]) Neurotoxicity - ([0160-4120]) Zebrafish - ([0160-4120]) Behavioral impairment - ([0160-4120]) Dopaminergic system - ([0160-4120]) Oxidative stress - ([0160-4120]) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Artículo científico indizado

Glyphosate is the active ingredient of some of the most highly produced and used herbicides worldwide. The intensive applications of glyphosate-based herbicides and its half-life in water lead to its presence in many aquatic ecosystems. Whereas recent studies have reported neurotoxic effects of glyphosate including autism- related effects, most of them used extremely high (mg/L to g/L) concentrations, so it is still unclear if chronic, low environmentally relevant concentrations of this compound (ng/L to μg/L) can induce neurotoxicity. In this study we analyzed the neurotoxicity of glyphosate in adult zebrafish after waterborne exposure to environmentally relevant concentrations (0.3 and 3 μg/L) for two weeks. Our data showed that exposed fish presented a significant impairment of exploratory and social behaviors consistent with increased anxiety. The anterior brain of the exposed fish presented a significant increase in dopamine and serotonin levels, as well as in the DOPAC/dopamine and homovanillic acid/dopamine turnover ratios. Moreover, the expression of genes involved in the dopaminergic system, as th1, th2, comtb, and scl6a3 was downregulated. Finally, the brain of exposed fish presented a significant increase in the catalase and superoxide dismutase activities, with a concomitant decrease of glutathione stores. These changes in the antioxidant defense system are consistent with the observed increase in oxidative stress, reflected by the increase in the levels of lipid peroxidation in the brain. The presented results show that current glyphosate concentrations commonly found in many aquatic ecosystems may have detrimental consequences on fish survival by decreasing exploration of the environment or altering social interactions. Furthermore, as zebrafish is also a vertebrate model widely used in human neurobehavioral studies, these results are relevant not only for environmental risk assessment, but also for understanding the risk of chronic low-dose exposures on human health.

This work was supported by the Spanish Government with FEDER Funds (CTM2017-83242-R; D.R.) and the network of recognized research groups by the Catalan Government (2017 SGR_902). J.B. was supported by a Spanish fellowship PRE2018-083513. Mention of spe- cific products or trade names does not indicate endorsement by the US federal government.

Editor

Environment International

Fecha de publicación

18 de noviembre de 2020

Tipo de publicación

Artículo

Recurso de información

http://hdl.handle.net/20.500.11799/109892

Fuente

0160-4120

Idioma

Inglés

Relación

146

2021

https://doi.org/10.1016/j.envint.2020.106253

Audiencia

Estudiantes

Investigadores

Repositorio Orígen

REPOSITORIO INSTITUCIONAL DE LA UAEM

Descargas

232