Título
Una estrategia para detectar anomalías cromosómicas en niños con leucemia linfoblástica aguda. /
A Strategy to detect chromosomal abnormalities in children with acute lymphoblastic leukemia.
Autor
SILVIA PATRICIA PEREZ VERA
SARA FRIAS VAZQUEZ
JOSE MIGUEL BETANCOURT RULE
MARISA MUJICA SANCHEZ
ROBERTO RIVERA LUNA
ROCIO ORTIZ LOPEZ
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Aberraciones cromosómicas - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Técnicas de diagnóstico molecular - Métodos - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Leucemia - Linfoma linfoblástico de células precursoras - Genética - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Chromosome aberrations - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Molecular diagnostic techniques - Methods - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Precursor cell lymphoblastic leukemia-Lymphoma - Genetics - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Anomalías cromosómicas - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Leucemia linfoblástica aguda - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Contenido de ADN - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Citometría de flujo - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) La fluorescencia de hibridación in situ - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Chromosomal abnormalities - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Acute lymphoblastic leukemia - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) DNA content - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Flow cytometry - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300]) Fluorescence in situ hybridization - ([Journal of Pediatric Hematology Oncology 26(5):294 - 300])
Resumen o descripción
Conventional cytogenetics (CC) can be used to identify chromosomal abnormalities that are predictors of treatment outcome in acute lymphoblastic leukemia (ALL). The detection of abnormalities in ALL is difficult because low mitotic index and poor-quality metaphases are obtained. Flow cytometry (FC) and fluorescence in situ hybridization (FISH) can be used to detect aneuploidy in any phaseofthecellcycle,increasingthenumberofanalyzablecells.The aim of this study was to develop a strategy combining these methods toimprovethefrequencyofchromosomeabnormalitydetection.One hundred children with newly diagnosed ALL were included. CC and DNA content analysis by FC were performed in all patients. The numericalabnormalitiesidentifiedbybothmethodswerecomparedand patientswereclassifiedasconcordantordiscordant.FISHwasusedto support aneuploidy results in discrepant cases using centromeric probesforthechromosomesmostfrequentlyinvolvedinaneuploidy. CC and FC showed high concordance (86%). Fourteen cases were discrepant: nine showed hypodiploidy and low hyperdiploidy by cytogenetics and five showed high hyperdiploidy by FC. FISH confirmed aneuploidy in 12 cases in which it could be performed. High hyperdiploidywasthemostcommonabnormality;the31casesshowing this aneuploidy were identified by FC. The search for abnormalities must begin by measuring DNA content to detect this aneuploidy, whichisusefultoevaluatethepatient’srisk.However,itisimportant toscreenforstructuralabnormalitiesbyCCormoleculartechniques. This strategy may detect chromosomal abnormalities, optimizing resourcesinlaboratorieswherenotallthescreeningmethodsareavailable.
Editor
Lippincott, Williams
&
Wilkins
Fecha de publicación
2004
Tipo de publicación
Artículo
Recurso de información
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3135
10.1097/00043426-200405000-00007
Formato
application/pdf
Fuente
Journal of Pediatric Hematology Oncology 26(5):294 - 300
Idioma
Inglés
Repositorio Orígen
Repositorio del Instituto Nacional de Pediatría
Descargas
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