Título
The participation of the muscarinic receptors in the preoptic-anterior hypothalamic areas in the regulation of ovulation depends on the ovary
Autor
FLORENCIA ADRIANA ESPINOSA VALDEZ
MAURA ANGELICA FLORES AGUILAR
ISABEL ARRIETA CRUZ
MARIO CARDENAS LEON
DAVID ROBERTO CHAVIRA RAMIREZ
ROBERTO DOMINGUEZ CASALA
MARIA ESTHER CRUZ BELTRAN
Nivel de Acceso
Acceso Abierto
Materias
MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Ciencias médicas - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Farmacodinámica - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Compuestos heterocíclicos - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Alcaloides solanáceos - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Alcaloides de belladona - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Derivados de atropina - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Atropina - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Procedimientos quirúrgicos operativos - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Procedimientos quirúrgicos urogenitales - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Castración - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Ovariectomía - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Ovulación - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Farmacodynamic - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Heterocyclic compounds - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Solanaceous alkaloids - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Belladonna alkaloids - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Atropine derivatives - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Atropine - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Surgical procedures, operative - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Urogenital surgical procedures - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Castration - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Ovariectomy - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)]) Ovulation - ([Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)])
Resumen o descripción
Abstract:
Background
Muscarinic receptors (mAChRs) of the preoptic and anterior hypothalamus areas (POA-AHA) regulate ovulation in an asymmetric manner during the estrous cycle. The aims of the present study were to analyze the effects of a temporal blockade of mAChRs on either side of the POA-AHA performed in diestrus-2 rats on ovulation, the levels of estradiol, follicle stimulating hormone (FSH) and luteinizing hormone (LH) and the mechanisms involved in changes in ovulation.
Methods
Cyclic rats on diestrus-2 day were anesthetized and randomly assigned to the following groups: 1) microinjection of 1 μl of saline or atropine solution (62.5 ng) in the left or right POA-AHA; 2) removal (unilateral ovariectomty, ULO) of the left (L-ULO) or right (R-ULO) ovary, and 3) rats microinjected with atropine into the left or right POA-AHA plus L-ULO or R-ULO. The ovulation rate and the number of ova shed were measured during the predicted estrus, as well as the levels of estradiol, FSH and LH during the predicted proestrus and the effects of injecting synthetic LH-releasing hormone (LHRH) or estradiol benzoate (EB).
Results
Atropine in the left POA-AHA decreased both the ovulation rate and estradiol and LH levels on the afternoon of proestrus, also LHRH or EB injection restored ovulation. L- or R-ULO resulted in a lower ovulation rate and smaller number of ova shed, and only injection of LHRH restored ovulation. EB injection at diestrus-2 restored ovulation in animals with L-ULO only. The levels of estradiol, FSH and LH in rats with L-ULO were higher than in animals with unilateral laparotomy. In the group microinjected with atropine in the left POA-AHA, ovulation was similar to that in ULO rats. In contrast, atropine in the right POA-AHA of ULO rats blocked ovulation, an action that was restored by either LHRH or EB injection.
Conclusions: These results indicated that the removal of a single ovary at noon on diestrus-2 day perturbed the neuronal pathways regulating LH secretion, which was mediated by the muscarinic system connecting the right POA-AHA and the ovaries.
Editor
BioMed Central
Fecha de publicación
2016
Tipo de publicación
Artículo
Recurso de información
Formato
Adobe PDF
application/pdf
Fuente
Reproductive Biology and Endocrinology (1477-7827) vol. 14 (2016)
Idioma
Inglés
Relación
https://rbej.biomedcentral.com/articles/10.1186/s12958-016-0208-3
Repositorio Orígen
INSTITUTO NACIONAL DE GERIATRIA
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