Título
Synthesis of keratine, silver, and flavonols nanocomposites to inhibit oxidative stress in pancreatic beta-cell (INS-1) and reduce intracellular reactive oxygen species production
Autor
ROSA MARTHA PEREZ GUTIERREZ
ISIS SCHERAZADA RODRIGUEZ CLAVEL
SILVIA PATRICIA PAREDES CARRERA
JESUS CARLOS SANCHEZ OCHOA
ALETHIA MUÑIZ RAMIREZ
SUSANNA EDITH MEDELLIN GARIBAY
ERI JOEL PAZ GARCIA
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1016/j.arabjc.2020.102917
Materias
Resumen o descripción
"Flavonols (FLA) from Vaccinium macrocarpon (V. macrocarpon) were identified using high-performance liquid chromatography coupled with mass spectrometry detection. Nanoparticles were prepared using highly crosslinked keratin (KER) from human hair and silver and entrapped with flavonols [KER + FLA + AgNPs]. Nanocomposites were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction, zeta potential, and dynamic light scattering, and release profiles. The interactions between the capping agent and the silver core have been investigated using FTIR spectroscopy H2O2 is a source of Reactive Oxygen Species (ROS) and acts as an activator of oxidative stress affecting NS-1 cells, and the protective effect of the nanocomposites were evaluated against H2O2-induced pancreatic beta-cell damage. LC-MS/MS and HPLC analyses revealed the presence of 12 flavonols in V. macrocarpon plant extract. The cell apoptosis and proliferation, were evaluated by Hoechst 33342 staining, flow cytometry and Cell Counting Kit-8 respectively. Preincubation of the NS-1 cells with 250 mu g/mL of H2O2 induced oxidative stress conditions that show pancreatic beta-cell dysfunction, including ROS, cell death, mitochondrial function, antioxidant enzymes, and lipid peroxidation. Nevertheless, pretreatment with FLA and [KER + FLA + AgNPs] prevented mitochondria disruption, maintained cellular ATP levels, inhibited LDH release, intracellular ROS production, decreased lipid peroxidation, increased expression of antioxidant enzymes (CAT, SOD, and GPx) and GSH levels. These results indicate that nanocomposites could protect rat INS-1 pancreatic beta-cell from H2O2-induced oxidative damage, apoptosis and proliferation by reducing the production of intracellular reactive oxygen species."
Editor
Elsevier
Fecha de publicación
2021
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Rosa Martha Pérez-Gutierrez, Isis Sherazada Rodríguez-Clavel, Silvia Patricia Paredes-Carrera, Jesus Carlos Sánchez-Ochoa, Alethia Muñiz-Ramirez, Susanna Medellin Garibay, Eri Joel Paz-García, Synthesis of keratine, silver, and flavonols nanocomposites to inhibit oxidative stress in pancreatic beta-cell (INS-1) and reduce intracellular reactive oxygen species production, Arabian Journal of Chemistry, Volume 14, Issue 2, 2021, 102917, https://doi.org/10.1016/j.arabjc.2020.102917.
Repositorio Orígen
Repositorio IPICYT
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