Título
Diisononyl Phthalate differentially affects sirtuin expression in the HepG2 cell line
Autor
ANA KAREN GUTIERREZ GARCIA
Mahua Choudhury
Antonio de León Rodríguez
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1021/acs.chemrestox.9b00206
Materias
Resumen o descripción
"Human exposure to phthalates has received special attention due to their possible adverse human health effects. Diisononyl phthalate (DINP) is a plasticizer still widely used in many products, despite being considered an endocrine disruptor. In this study, we evaluated DINP’s cytotoxicity, its effect on the levels of reactive oxygen species (ROS), and its effect on sirtuin expression in HepG2 cells. Results showed that 1 μg/mL DINP significantly downregulated Sirt1, Sirt2, Sirt3, and Sirt5 gene expression (p < 0.05), while other sirtuins remained unaffected. Furthermore, protein levels of Sirt1 and Sirt3 were significantly downregulated by 1 μg/mL DINP. On the other hand, 100 μg/mL DINP doubled the levels of lysine acetylation proteins (increased 2-fold) as well as reactive oxygen species (ROS) compared with the controls. In conclusion, our study suggests, for the first time, that DINP regulates the potential epigenetic disruptor sirtuin family and leads to induction of ROS via sirtuins."
Editor
American Chemical Society
Fecha de publicación
2019
Tipo de publicación
Artículo
Versión de la publicación
Versión enviada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Gutiérrez-García AK, Choudhury M, De Leon-Rodriguez A. Diisononyl Phthalate Differentially Affects Sirtuin Expression in the HepG2 Cell Line. Chem Res Toxicol. 2019 Sep 16;32(9):1863-1870. doi: 10.1021/acs.chemrestox.9b00206.
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Repositorio IPICYT
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