Título

Diversification of the kinetic properties of yeast NADP‐glutamate‐dehydrogenase isozymes proceeds independently of their evolutionary origin

Autor

JOSE CARLOS CAMPERO BASALDUA

Hector Quezada

LINA RAQUEL RIEGO RUIZ

DARIEL MARQUEZ GUTIERREZ

James González

MOHAMMED EL HAFIDI BENTLAKDER

MARIA ALICIA GONZALEZ MANJARREZ

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1002/mbo3.419

Materias

Functional diversification - (AUTOR) Glutamate dehydrogenase - (AUTOR) Kinetics - (AUTOR) Paralogous enzymes - (AUTOR) Phylogeny - (AUTOR) Yeast gene duplication - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) MICROBIOLOGÍA - (CTI)

Resumen o descripción

"In the yeast Saccharomyces cerevisiae, the ScGDH1 and ScGDH3 encoded glutamate dehydrogenases (NADP‐GDHs) catalyze the synthesis of glutamate from ammonium and α‐ketoglutarate (α‐KG). Previous kinetic characterization showed that these enzymes displayed different allosteric properties and respectively high or low rate of α‐KG utilization. Accordingly, the coordinated action of ScGdh1 and ScGdh3, regulated balanced α‐KG utilization for glutamate biosynthesis under either fermentative or respiratory conditions, safeguarding energy provision. Here, we have addressed the question of whether there is a correlation between the regulation and kinetic properties of the NADP‐GDH isozymes present in S. cerevisiae (ScGdh1 and ScGdh3), Kluyveromyces lactis (KlGdh1), and Lachancea kluyveri (LkGdh1) and their evolutionary history. Our results show that the kinetic properties of K. lactis and L. kluyveri single NADP‐GDHs are respectively similar to either ScGDH3 or ScGDH1, which arose from the whole genome duplication event of the S. cerevisiae lineage, although, KlGDH1 and LkGDH1 originated from a GDH clade, through an ancient interspecies hybridization event that preceded the divergence between the Saccharomyces clade and the one containing the genera Kluyveromyces, Lachancea, and Eremothecium. Thus, the kinetic properties which determine the NADP‐GDHs capacity to utilize α‐KG and synthesize glutamate do not correlate with their evolutionary origin."

Editor

John Wiley

&

Sons

Fecha de publicación

abril de 2017

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1631

Formato

application/pdf

Sugerencia de citación

Campero‐Basaldua, C., Quezada, H., Riego‐Ruíz, L., Márquez, D., Rojas, E., González, J., El‐Hafidi, M. and González, A. Diversification of the kinetic properties of yeast NADP‐glutamate‐dehydrogenase isozymes proceeds independently of their evolutionary origin. MicrobiologyOpen. 2017;6:e00419. https://doi.org/10.1002/mbo3.419

Repositorio Orígen

Repositorio IPICYT

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