Título

Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites

Autor

Antonio de León Rodríguez

Santiago L Caño Muñiza

Junyang Liu

David K. Summers

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1016/j.nbt.2016.09.005

Materias

Bacteriostatic - (AUTOR) Escherichia coli - (AUTOR) Hydrogen - (AUTOR) Ethanol - (AUTOR) Indole - (AUTOR) Ionophore - (AUTOR) Lactate - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR - (CTI)

Resumen o descripción

"Indole is a bicyclic signaling molecule with effects on both eukaryotic and prokaryotic cells. The majority of studies of indole action have been performed with bacteria cultured under aerobic conditions and little information is available about its effects under anaerobic conditions. Here the effect of the indole on anaerobic metabolism of Escherichia coli WDHL was studied. Indole in the range 0.5–8 mM was added to the culture medium and cell growth, hydrogen and metabolite production were compared to cultures lacking indole. Results showed that while 8 mM indole abolished growth completely, 4 mM indole had a partial bacteriostatic effect and the maximum optical density of the culture decreased by 44% compared to the control cultures. In addition, 4 mM indole had an important effect on anaerobic metabolism. Hydrogen production increased from 650 ± 115 to 1137 ± 343 mL H2/L, and hydrogen yield increased from 0.45 ± 0.1 to 0.94 ± 0.34 mol H2/mol glucose, compared to the control culture. Carbon flux was also affected and the composition of the final by-products changed. Lactate (41 mM) was the main metabolite in the control cultures, whereas ethanol (56.2 mM) and acetate (41.2 mM) were the main metabolites in the cultures with 2 mM indole. We conclude that the supplementation of E. coli cultures with exogenous indole is a simple and novel strategy to improve the production of hydrogen as well as other metabolites such as ethanol used as biofuels."

Editor

Elsevier Science BV

Fecha de publicación

diciembre de 2016

Tipo de publicación

Artículo

Versión de la publicación

Versión enviada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1491

Formato

application/pdf

Sugerencia de citación

Antonio De Leon-Rodriguez, Santiago L. Caño-Muñiz, Junyan Liu, David K. Summers, Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites, New Biotechnology, Volume 33, Issue 6, 2016, Pages 868-873, ISSN 1871-6784.

Repositorio Orígen

Repositorio IPICYT

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