Título
Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate
Autor
Zazil Donaxí Alvarado Cuevas
LEANDRO GABRIEL ORDOÑEZ ACEVEDO
JOSE TOMAS ORNELAS SALAS
Antonio de León Rodríguez
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1016/j.nbt.2013.03.002
Materias
Resumen o descripción
"The impact of nitrogen source on hydrogen production by Escherichia coli WDHL (Delta hycA Delta lacI) strain using cheese whey as a substrate was evaluated. To improve the assimilation of complex proteins such as lactalbumin, we assessed treatment with a protease. Also, five external nitrogen sources were tested: NH4Cl, (NH4)(2)SO4, urea, yeast extract, and tryptone. The treatments in 120 mL serological bottles with pancreatin 1000 mg/L produced 1.75-fold more hydrogen than the cultures without pancreatin. In the bottle cultures supplemented with yeast extract or tryptone 5 g/L, hydrogen production increased up to 3.2- and 3.5-fold, respectively, whereas inorganic salts and urea had no statistical difference with respect to the control cultures. In 1-L bioreactors, the use of tryptone improved 2.1-fold hydrogen production. Tryptone or yeast extract enable the total consumption of lactose in 40 h, whereas in the control assay the lactose was not completely consumed. Our results demonstrate that it is necessary to select an adequate nitrogen source, which allows both carbon source consumption and high hydrogen production."
Editor
Elsevier Science BV
Fecha de publicación
septiembre de 2013
Tipo de publicación
Artículo
Versión de la publicación
Versión enviada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Zazil D. Alvarado-Cuevas, Leandro G. Ordoñez Acevedo, José Tomás Ornelas Salas, Antonio De León-Rodríguez, Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate, New Biotechnology, Volume 30, Issue 6, 2013, Pages 585-590.
Repositorio Orígen
Repositorio IPICYT
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