Título

Identification of calcium stress induced genes in amaranth leaves through suppression subtractive hybridization

Autor

Hugo Sergio Aguilar Hernández

MARTHA LETICIA SANTOS MARTINEZ

Ma. Fabiola León Galván

Alberto Barrera Pacheco

Eduardo Espitia Rangel

Antonio de León Rodríguez

Ramón Gerardo Guevara González

Ana Paulina Barba de la Rosa

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1016/j.jplph.2011.06.006

Materias

Amaranthus hypochondriacus - (AUTOR) Calcium stress - (AUTOR) Subtractive cDNA libraries - (AUTOR) Zinc finger protein - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA VEGETAL (BOTÁNICA) - (CTI) BIOLOGÍA VEGETAL (BOTÁNICA) - (CTI)

Resumen o descripción

"Calcium (Ca2+) is a critical ion for the growth and development of plants and plays an important role in signal transduction pathways in response to biotic and abiotic stresses. We investigated the Ca2+ stress responsive-genes in amaranth leaves by using the suppression subtractive hybridization technique. Screening of the libraries generated 420 up-regulated transcripts and 199 down-regulated transcripts. The differentially expressed transcripts were associated with general stress response, transcription factors, gene regulation, signal transduction, and some other with unknown function. Selected genes were used to study their differential regulation by sqRT-PCR. Among the up-regulated transcripts, a fragment containing the motif of C3HC4-type RING-Zinc family was further characterized. The ORF of amaranth zinc finger protein (AhZnf) has a closer relationship with its ortholog from Ricinus communis while is distantly related to the Arabidopsis thaliana C3HC4-type ortholog. We have identified a novel putative zinc finger protein along with other novel proteins such as the wall associated kinase, phosphoinositide binding protein, and rhomboid protease involved in response to Ca2+ stress in amaranth leaves."

Editor

Elsevier B.V.

Fecha de publicación

noviembre de 2011

Tipo de publicación

Artículo

Versión de la publicación

Versión aceptada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1480

Formato

application/pdf

Sugerencia de citación

Hugo S. Aguilar-Hernández, Leticia Santos, Fabiola León-Galván, Alberto Barrera-Pacheco, Eduardo Espitia-Rangel, Antonio De León-Rodríguez, Ramón G. Guevara-González, Ana P. Barba de la Rosa, Identification of calcium stress induced genes in amaranth leaves through suppression subtractive hybridization, Journal of Plant Physiology, Volume 168, Issue 17, 2011, Pages 2102-2109.

Repositorio Orígen

Repositorio IPICYT

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