Título
Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate
Autor
Luis Manuel Rosales Colunga
Elías Razo Flores
LEANDRO GABRIEL ORDOÑEZ ACEVEDO
Felipe Alatriste Mondragón
Antonio de León Rodríguez
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1016/j.ijhydene.2009.10.097
Materias
Resumen o descripción
"This study reports a fermentative hydrogen production by Escherichia coli using cheese whey as substrate. To improve the biohydrogen production, an E. coli ΔhycA ΔlacI strain (WDHL) was constructed. The absence of hycA and lacI genes had a positive effect on the biohydrogen production. The strain produced 22% more biohydrogen in a shorter time than the wild-type (WT) strain. A Box-Behnken experimental design was used to optimize pH, temperature and substrate concentration. The optimal initial conditions for biohydrogen production by WDHL strain were pH 7.5, 37 °C and 20 g/L of cheese whey. The specific production rate was improved from 3.29 mL H2/optical density at 600 nm (OD600nm) unit-h produced by WDHL under non-optimal conditions to 5.88 mL H2/OD600nm unit-h under optimal conditions. Using optimal initial conditions, galactose can be metabolized by WDHL strain. The maximum yield obtained was 2.74 mol H2/mol lactose consumed, which is comparable with the yield reached in other hydrogen production processes with Clostridium sp. or mixed cultures."
Editor
Elsevier B.V
Fecha de publicación
enero de 2010
Tipo de publicación
Artículo
Versión de la publicación
Versión aceptada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Luis Manuel Rosales-Colunga, Elías Razo-Flores, Leandro G. Ordoñez, Felipe Alatriste-Mondragón, Antonio De León-Rodríguez, Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate, International Journal of Hydrogen Energy, Volume 35, Issue 2, 2010, Pages 491-499.
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Repositorio IPICYT
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