Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate


Luis Manuel Rosales Colunga

Elías Razo Flores


Felipe Alatriste Mondragón

Antonio de León Rodríguez

Access level

Open Access

Alternative identifier

doi: https://doi.org/10.1016/j.ijhydene.2009.10.097

Summary or description

"This study reports a fermentative hydrogen production by Escherichia coli using cheese whey as substrate. To improve the biohydrogen production, an E. coli ΔhycA ΔlacI strain (WDHL) was constructed. The absence of hycA and lacI genes had a positive effect on the biohydrogen production. The strain produced 22% more biohydrogen in a shorter time than the wild-type (WT) strain. A Box-Behnken experimental design was used to optimize pH, temperature and substrate concentration. The optimal initial conditions for biohydrogen production by WDHL strain were pH 7.5, 37 °C and 20 g/L of cheese whey. The specific production rate was improved from 3.29 mL H2/optical density at 600 nm (OD600nm) unit-h produced by WDHL under non-optimal conditions to 5.88 mL H2/OD600nm unit-h under optimal conditions. Using optimal initial conditions, galactose can be metabolized by WDHL strain. The maximum yield obtained was 2.74 mol H2/mol lactose consumed, which is comparable with the yield reached in other hydrogen production processes with Clostridium sp. or mixed cultures."


Elsevier B.V

Publish date

January, 2010

Publication type


Publication version

Accepted Version



Citation suggestion

Luis Manuel Rosales-Colunga, Elías Razo-Flores, Leandro G. Ordoñez, Felipe Alatriste-Mondragón, Antonio De León-Rodríguez, Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate, International Journal of Hydrogen Energy, Volume 35, Issue 2, 2010, Pages 491-499.

Source repository

Repositorio IPICYT




You need to sign in or sign up to comment.