Título

Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

Autor

Emilio Medina Rivero

VICTOR EMMANUEL BALDERAS HERNANDEZ

LEANDRO GABRIEL ORDOÑEZ ACEVEDO

Luz María Teresita Paz Maldonado

Ana Paulina Barba de la Rosa

Antonio de León Rodríguez

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: https://doi.org/10.1007/s10529-007-9395-5

Materias

Periplasm - (AUTOR) Signal peptide - (AUTOR) Tat pathway - (AUTOR) Therapeutic protein - (AUTOR) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI) CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA - (CTI)

Resumen o descripción

"Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction."

Editor

Springer

Fecha de publicación

septiembre de 2007

Tipo de publicación

Artículo

Versión de la publicación

Versión aceptada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1468

Formato

application/pdf

Sugerencia de citación

Medina-Rivero, E., Balderas-Hernández, V.E., Ordoñez-Acevedo, L.G. et al. Biotechnol Lett (2007) 29: 1369. https://doi.org/10.1007/s10529-007-9395-5

Repositorio Orígen

Repositorio IPICYT

Descargas

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