Título
Directing the self-assembly of tumour spheroids by bioprinting cellular heterogeneous models within alginate/gelatin hydrogels
Autor
Tao Jiang
José Gil Munguía López
SALVADOR FLORES TORRES
Joel Grant
Sanahan Vijayakumar
Antonio de León Rodríguez
Joseph Kinsella
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1038/s41598-017-04691-9
Materias
Resumen o descripción
"Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After similar to 15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue coculture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks."
Editor
Nature Publishing Group
Fecha de publicación
julio de 2017
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Jiang, T., Munguia-Lopez, J.G., Flores-Torres, S. et al. Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels. Sci Rep 7, 4575 (2017). https://doi.org/10.1038/s41598-017-04691-9
Repositorio Orígen
Repositorio IPICYT
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