Título

Directing the self-assembly of tumour spheroids by bioprinting cellular heterogeneous models within alginate/gelatin hydrogels

Autor

Tao Jiang

José Gil Munguía López

SALVADOR FLORES TORRES

Joel Grant

Sanahan Vijayakumar

Antonio de León Rodríguez

Joseph Kinsella

Nivel de Acceso

Acceso Abierto

Identificador alterno

doi: https://doi.org/10.1038/s41598-017-04691-9

Resumen o descripción

"Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After similar to 15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue coculture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks."

Editor

Nature Publishing Group

Fecha de publicación

julio de 2017

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Sugerencia de citación

Jiang, T., Munguia-Lopez, J.G., Flores-Torres, S. et al. Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels. Sci Rep 7, 4575 (2017). https://doi.org/10.1038/s41598-017-04691-9

Repositorio Orígen

Repositorio IPICYT

Descargas

1097

Comentarios



Necesitas iniciar sesión o registrarte para comentar.