Directing the self-assembly of tumour spheroids by bioprinting cellular heterogeneous models within alginate/gelatin hydrogels


Tao Jiang

José Gil Munguía López


Joel Grant

Sanahan Vijayakumar

Antonio de León Rodríguez

Joseph Kinsella

Access level

Open Access

Alternative identifier

doi: https://doi.org/10.1038/s41598-017-04691-9

Summary or description

"Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After similar to 15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue coculture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks."


Nature Publishing Group

Publish date

July, 2017

Publication type


Publication version

Published Version



Citation suggestion

Jiang, T., Munguia-Lopez, J.G., Flores-Torres, S. et al. Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels. Sci Rep 7, 4575 (2017). https://doi.org/10.1038/s41598-017-04691-9

Source repository

Repositorio IPICYT




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