Título

High affinity promoter binding of STOP1 is essential for early expression of novel aluminum-induced resistance genes GDH1 and GDH2 in Arabidopsis

Autor

JAVIER MORA MACIAS

DAGOBERTO ARMENTA MEDINA

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-sa/4.0

Referencia de datos

datasetDOI/https://academic.oup.com/jxb/article/72/7/2769/6106577

Materias

Acid soils--Tropics - (LIBRARY OF CONGRESS AUTHORITY) Arabidopsis thaliana - (LIBRARY OF CONGRESS AUTHORITY) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) GENÉTICA - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI)

Resumen o descripción

Malate efflux from roots, which is regulated by the transcription factor STOP1 (SENSITIVE-TO-PROTON-RHIZOTOXICITY1) and mediates aluminum-induced expression of ALUMINUM-ACTIVATED-MALATE-TRANSPORTER1 (AtALMT1), is critical for aluminum resistance in Arabidopsis thaliana. Several studies showed that AtALMT1 expression in roots is rapidly observed in response to aluminum; this early induction is an important mechanism to immediately protect roots from aluminum toxicity. Identifying the molecular mechanisms that underlie rapid aluminum resistance responses should lead to a better understanding of plant aluminum sensing and signal transduction mechanisms. In this study, we observed that GFP-tagged STOP1 proteins accumulated in the nucleus soon after aluminum treatment. The rapid aluminum-induced STOP1-nuclear localization and AtALMT1 induction were detected in the presence of a protein synthesis inhibitor, suggesting that post-translational regulation is involved in these events. STOP1 also regulated rapid aluminum-induced expression for other genes that carry a functional/high-affinity STOP1-binding site in their promoter, including STOP2, GLUTAMATE-DEHYDROGENASE1 and 2 (GDH1 and 2). However STOP1 did not regulate Al resistance genes which have no functional STOP1-binding site such as ALUMINUM-SENSITIVE3, suggesting that the binding of STOP1 in the promoter is essential for early induction. Finally, we report that GDH1 and 2 which are targets of STOP1, are novel aluminum-resistance genes in Arabidopsis.

Editor

Journal of Experimental Botany

INFOTEC Centro de Investigación e Innovación en Tecnologías de la Información y Comunicación

Fecha de publicación

22 de enero de 2021

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://infotec.repositorioinstitucional.mx/jspui/handle/1027/521

Formato

application/pdf

Fuente

Mutsutomo Tokizawa, Dagoberto Armenta Medina, Javier Mora-Macías. (2021). La unión del promotor de alta afinidad de STOP1 es esencial para la expresión temprana de los nuevos genes de resistencia inducida por alumi

Idioma

Inglés

Sugerencia de citación

Normas APA 7.ª edición

Repositorio Orígen

Repositorio Institucional de INFOTEC

Descargas

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