Título
Validation of method for the separation and quantification of Arsenic species in human urine samples by HPLC/ICP-MS
Autor
LUZ OLIVIA LEAL QUEZADA
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
The importance of arsenic speciation is being increasingly recognized by the scientific community because intrinsic
arsenic species are known to exhibit differences in mobility, bioavailability and toxicity. A variety of organic and
inorganic species have been identified in environmental and biological samples. The aim of this study was to determine
the optimal operating parameters for chemical speciation of arsenic in human urine samples. HPLC/ICP-MS technique
was employed to determine arsenobetaine (AsB), dimethyl arsenic acid (DMA), monomethyl arsenic acid (MMA),
arsenite [As (III)] and arsenate [As (V)] in human urine samples. The separation was carried out on the Hamilton PRP
X100 column, using two mobile phases: phase A containing 10 mM ammonium carbonate and phase B containing 20 mM
ammonium carbonate at pH 8.83-9.35. The performance of the method was evaluated in terms of linearity, precision,
accuracy and detection and quantification limits. The results demonstrate that the HPLC/ICP-MS methodology proposed
is a sensitive, reproducible and accurate technique for the determination of arsenic species at trace levels in human
urine.
Fecha de publicación
2011
Tipo de publicación
Memoria de congreso
Versión de la publicación
Versión enviada
Recurso de información
Formato
application/pdf
Idioma
Inglés
Repositorio Orígen
Fuente de Objetos Científicos Open Access
Descargas
192