Título
Isolation and Characterization of a Soluble Phosphate Hydrolysing Activity from an in vitro Coffee Cell Line Grown in the Presence of Aluminum
Autor
YERENI MINERO GARCÍA
MARCO ANTONIO VILLANUEVA MENDEZ
FELIPE AUGUSTO VAZQUEZ FLOTA
SOLEDAD MARIA TERESA HERNANDEZ SOTOMAYOR
IGNACIO RODRIGO ISLAS FLORES
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
As our interest is focused in obtaining a betler knowledge of the plant phosphate metabolism and its interactions with Al ion, we used an in vitro coffee cellline (Coffia arabica L.) tolerant to allllIlimun ion to purify a protein with phosphate hydrolysing activity with the airn to analyze whether the enzyrne can be contributing to the Al-tolerance in this coffee cellline. The protein was purified at least 138-fold; silver stain on a 10% SDS-PAGE detected a partially purified 30 kDa polypeptide which showed ability to hydrolyse phosphate either in native gels or soluble assays. The semipurified protein is able to hydrolyse sodiurn pyrophosphate (PPi-Na) and adenosine triphosphate (ATP) very efficiently, a1lhough P-serine (P-ser), P-Threonine (P-thr), P-Tyrosine (P-Tyr), Phytate, D-Myo-lnositol-lP (D-Myo Inos-lP) and lhe synlhetic p-nyliophenyl phosphate (P-NPP) could also be used as substrates. Enzyrnatic activity ofthe EDTA-inactivated enzyrne can be restored by Mg2+, as well as other divalent cations such as Fe2+, Co2+, Cu2+, Zn2+, Ca2+ and Mn2+. In contrast, Al3+ could only partially reactivate the phosphate hydrolysing activity, which suggests that it is not a cofactor for this enzyrne. Vv'hen Al3 + was added to the Mg2+ -enzyrne complex, it strongly inhibited the enzyrnatic activity either, exerting a negative effect over the enzyrne or the substrate. Between a munber of phosphohydrolase inhibitors, only KN03 was able to decrease the activity suggesting that this enzyme should be related with the V-ATPase protein family or with plant phosphatases.
Fecha de publicación
2007
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Asian Journal of Biochemistry. 2. 302-313. 10.3923/ajb.2007.302.313.
Idioma
Inglés
Relación
&
Villanueva, Marco
&
Vazquez-Flota, Felipe
&
S.M. Teresa, Hernandez-Sotomayor
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Islas-Flores, Ignacio. (2007). Isolation and Characterization of a Soluble Phosphate Hydrolysing Activity from an in vitro Coffee Cell Line Grown in the Presence of Aluminum. Asian Journal of Biochemistry. 2. 302-313. 10.3923/ajb.2007.302.313.
Sugerencia de citación
Yereni, Minero-Garcia
Repositorio Orígen
Repositorio Institucional CICY
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