Título
Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)
Autor
NANCY SANTANA BUZZY
Colaborador
RAUL ENRIQUE VALLE GOUGH (Colaborador)
SUSANA ALEJANDRA AVILES VINAS (Colaborador)
STEPHANIE LOPEZ EROSA (Colaborador)
ADRIANA CANTO FLICK (Colaborador)
EUNICE GOMEZ UC (Colaborador)
LUIS ALFONSO SAENZ CARBONELL (Colaborador)
NEFTALI OCHOA ALEJO (Colaborador)
Nivel de Acceso
Acceso Abierto
Referencia de datos
doi: DOI: 10.5897/AJB2014.14313/
Materias
Resumen o descripción
In order to determine the role of polyamines in the formation and development of the somatic embryos of Capsicum chinense, the effect of different concentrations (0, 0.01, 0.1, and 1.0 mM) of Putrescine, Spermidine and Spermine on the efficiency and morphology of the embryos was evaluated. The results show that none of the three polyamines evaluated had a significant effect on the number of embryos formed, except Spermidine (1 mM), which caused a significant reduction in their numbers, in comparison with the control treatment. However, the most noteworthy result was observed in the treatment containing 0.1 mM of Spermine. The embryos developed in this treatment showed harmonic apex-radicle development, pale-green coloration and the formation of two tiny cotyledonary leaves. Real-time PCR was used to analyze the differential expression of the WUS, WOX1 and WOX3 genes in somatic embryos treated with Spermine and untreated, including the zygotic embryo. The transcript levels of the genes analyzed were found to differ significantly between both types of embryos (somatic and zygotic), with the zygotic embryos presenting a higher level of transcripts; however, compared to the untreated somatic embryos, the somatic embryos treated with Spermine showed an increase in the transcript levels of the three genes analyzed (WUS, WOX1 and WOX3); the WOX1 gene in particular presented an accumulation pattern similar to that of the zygotic embryo of the species.
Fecha de publicación
28 de febrero de 2015
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
African Journal of Biotechnology. 14(7), 569-581, 2015
Idioma
Inglés
Relación
&
Santana-Buzzy, N. (2015). Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.). African Journal of Biotechnology, 14(7), 569-581.
Sugerencia de citación
Valle-Gough, R. E., Avilés-Viñas, S. A., López-Erosa, S., Canto-Flick, A., Gómez-Uc, E., Sáenz-Carbonell, L. A., ...
Repositorio Orígen
Repositorio Institucional CICY
Descargas
378