Título
Somaclonal Variation in Habanero Pepper (Capsicum chinense Jacq.) as Assessed ISSR Molecular Markers
Autor
NANCY SANTANA BUZZY
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of
in vitro culture on genetic variation in Habanero pepper (Capsicum chinense Jacq.)
regeneration protocols. A total of 219 ISSR clear and reproducible fragments were
generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic
embryos, and the embryogenic callus system. A cluster analysis was performed to express
in the form of dendrogram the relationships among different regeneration systems and
the genetic variability detected. Genetic distance analysis indicated that our regeneration
protocols are inappropriate for micropropagation, conservation, or genetic transformation;
however, they may be applicable to breeding. This is the first report on the use of
molecular analysis to evaluate genetic variation of in vitro-regenerated plants of
Habanero pepper using ISSR markers.
Fecha de publicación
5 de marzo de 2014
Tipo de publicación
Artículo
Recurso de información
Formato
application/pdf
Repositorio Orígen
Repositorio Institucional CICY
Descargas
1707