Título

Detection and identification of phytoplasmas in the 16SrIV-A,-B, and-D subgroups in palms in Tabasco, Mexico

Autor

EDER RAMOS HERNANDEZ

JULIA MARIA LESHER GORDILLO

Carlos Mariano Oropeza Salín

CARLOS FREDY ORTIZ GARCIA

MIGUEL ALBERTO MAGAÑA ALEJANDRO

SAUL SANCHEZ SOTO

YESENIA GARCIA ESTRADA

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/https://doi.org/10.1094/PDIS-09-18-1488-RE

Materias

16SRIV PHYTOPLASMAS - (AUTORES) RFLP - (AUTORES) PATHOSYSTEM - (AUTORES) HOST PLANTS - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR DE PLANTAS - (CTI) BIOLOGÍA MOLECULAR DE PLANTAS - (CTI)

Resumen o descripción

The 16SrIV-A phytoplasmas are associated with the devastating disease lethal yellowing (LY) of palms. In Tabasco (Mexico), the death of Cocos nucifera, Adonidia merrillii, and Attalea butyracea palms have been suspected to be associated with LY based on symptomatology. Samples from the trunk of both symptomatic and nonsymptomatic palms were collected in three different environments: two species of palms within a rural zone and the other within an urban zone. DNA was extracted to perform a nested PCR with phytoplasma primers P1/P7-LY16SF/R16R2. A 1,345-bp fragment was amplified from the DNA extracted from each of the 29 LY-symptomatic palms sampled. Phytoplasma identification was achieved by amplicon sequencing and virtual restriction fragment length polymorphism analyses. Three 16SrIV phytoplasma subgroups were detected: 16SrIV-A in C. nucifera, 16SrIV-B in A. merrillii, and 16SrIV-D in C. nucifera, A. merrillii, and A. butyracea. Phylogenetic analysis showed also that the three phytoplasma strains found in the palm species clustered with phytoplasmas reported in the literature in the three subgroups identified. This is the first report of phytoplasmas associated with these palm species in Tabasco.

Fecha de publicación

2020

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1936

Formato

application/pdf

Fuente

Plant Disease, 104(10), 2020, 2606-2612.

Idioma

Inglés

Relación

&

García Estrada, Y. (2020). Detection and identification of phytoplasmas in the 16SrIV-A,-B, and-D subgroups in palms in Tabasco, Mexico. Plant Disease, 104(10), 2606-2612.

Sugerencia de citación

Ramos Hernández, E., Lesher Gordillo, J. M., Oropeza Salín, C., Ortiz García, C. F., Magaña Alejandro, M. A., Sánchez Soto, S.,

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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