Título

Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)

Autor

SAMUEL DAVID GAMBOA TUZ

ALEJANDRO PEREIRA SANTANA

Jesús Alejandro Zamora Briseño

Enrique Castaño de la Serna

FRANCISCO LEONEL ESPADAS Y GIL

JORGE TONATIUH AYALA SUMUANO

MIGUEL ANGEL KEB LLANES

Lorenzo Felipe Sánchez Teyer

Luis Carlos Rodríguez Zapata

Nivel de Acceso

Acceso Abierto

Referencia de datos

datasetDOI/10.1038/s41598-018-32904-2

Resumen o descripción

Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.

Fecha de publicación

2018

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

Scientific reports, 8(1), 14539, 2018.

Idioma

Inglés

Relación

&

Rodríguez-Zapata, L. C. (2018). Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.). Scientific reports, 8(1), 14539.

Sugerencia de citación

Gamboa-Tuz, S. D., Pereira-Santana, A., Zamora-Briseño, J. A., Castano, E., Espadas-Gil, F., Ayala-Sumuano, J. T., ...

Repositorio Orígen

Repositorio Institucional CICY

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