Título
Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
Autor
SAMUEL DAVID GAMBOA TUZ
ALEJANDRO PEREIRA SANTANA
Jesús Alejandro Zamora Briseño
Enrique Castaño de la Serna
FRANCISCO LEONEL ESPADAS Y GIL
JORGE TONATIUH AYALA SUMUANO
MIGUEL ANGEL KEB LLANES
Lorenzo Felipe Sánchez Teyer
Luis Carlos Rodríguez Zapata
Nivel de Acceso
Acceso Abierto
Referencia de datos
datasetDOI/10.1038/s41598-018-32904-2
Materias
Resumen o descripción
Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.
Fecha de publicación
2018
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Scientific reports, 8(1), 14539, 2018.
Idioma
Inglés
Relación
&
Rodríguez-Zapata, L. C. (2018). Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.). Scientific reports, 8(1), 14539.
Sugerencia de citación
Gamboa-Tuz, S. D., Pereira-Santana, A., Zamora-Briseño, J. A., Castano, E., Espadas-Gil, F., Ayala-Sumuano, J. T., ...
Repositorio Orígen
Repositorio Institucional CICY
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