Título

Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae).

Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae).

Autor

NADIA GUADALUPE SANCHEZ COELLO

MAURICIO LUNA RODRIGUEZ

SANTIAGO MARIO VAZQUEZ TORRES

LAZARO RAFAEL SANCHEZ VELASQUEZ

NANCY SANTANA BUZZY

PABLO OCTAVIO AGUILAR

LOURDES GEORGINA IGLESIAS ANDREU

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/http://dx.doi.org/10.5154/r.rchscfa.2011.03.024

Materias

CERATOZAMIA MEXICANA - (AUTORES) CICADAS - (AUTORES) EXTRACCION DE ADN - (AUTORES) ESPECIES EN PELIGRO - (AUTORES) ISSR - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Most of the cycads contain high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA extraction, causing erroneous or no PCR products. The optimization of DNA isolation, employing inter-simple sequence repeats (ISSRs) primers were investigated in Ceratozamia mexicana Brongn., an endangered Mexican cycad. The DNA obtained from fresh-leaf tissues with a modified cetyltrimethylammonium bromide buffer protocol gave a good quality of DNA with no colored pigments and contaminants. The main modification to the CTAB-based DNA extraction protocol was the one hour leaf tissue soaking pre-treatment with a 0.7 M NaCl solution, to facilitate the cell lysis. The DNA extracted was successfully amplified by PCR using six arbitrary ISSR primers. Reproducible amplifiable products were observed in all PCR reactions. Our results show a significant improvement in the DNA quality obtained using low primer concentration (25 pM). 23 strong bands were detected, 9 of which were polymorphic. The results indicated that the optimized protocol for DNA isolation and PCR system is suitable for further work in this specie. This work is the first DNA extraction and ISSR protocols reported for this ornamental and endangered species.

Fecha de publicación

2012

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1264

Formato

application/pdf

Fuente

Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133, 2012.

Idioma

Español

Relación

&

Iglesias-Andreu, L. G. (2012). Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae). Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133.

Sugerencia de citación

Sánchez-Coello, N. G., Luna-Rodríguez, M., Vázquez-Torres, S. M., Sánchez-Velásquez, L. R., Santana-Buzzy, N., Octavio-Aguilar, P.,

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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