Título

Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis

Autor

YENY LIZZET COUOH UICAB

IGNACIO RODRIGO ISLAS FLORES

NUVIA EUGENIA KANTUN MORENO

MIGUEL ALONSO TZEC SIMA

SANTY PERAZA ECHEVERRIA

LIGIA GUADALUPE BRITO ARGAEZ

LETICIA PERAZA ECHEVERRIA

ROSA GRIJALVA ARANGO

ANDREW CHRISTOPHER JAMES

CECILIA MONICA RODRIGUEZ GARCIA

Nivel de Acceso

Acceso Abierto

Referencia de datos

doi: DOI: 10.5897/AJB11.2966

Resumen o descripción

ABC transporters are membrane proteins that use the energy released from the hydrolysis of ATP to drive the transport of compounds across biological membranes. In some plants, pathogenic fungi ABC transporters play a role as virulence factors by mediating the export of plant defense compounds or fungal virulence factors. Mycosphaerella fijiensis, the causal agent of black Sigatoka disease in banana, is the main constraint for the banana industry worldwide. So far, little is known about molecular mechanism that it uses to infect the host. In this study, degenerated primers designed from fungal ABC transporters known to be involved in virulence were used to isolate homologs from M. fijiensis.Here, we reported the full cloning of MfAtr4 a putative ortholog of MgAtr4, an ABC transporter of the related Mycosphaerella graminicola with a function in virulence. Similarities and differences with its presumed ortholog MgAtr4 are described, and the putative function of MfAtr4 are discussed. Analysis of MfAtr4 gene expression in field banana samples exhibiting visible symptoms of black Sigatoka disease indicated a higher expression of MfAtr4 during the first symptomatic stages in comparison to the late necrotrophic phases, suggesting a role for MfAtr4 in the early stages of pathogenic development of M. fijiensis.

Fecha de publicación

2012

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

 African Journal of Biotechnology, 11(1), 54-79, 2012

Idioma

Inglés

Relación

&

Rodríguez-García, C. (2012). Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis. African Journal of Biotechnology, 11(1), 54-79.

Sugerencia de citación

Couoh-Uicab, Y., Islas-Flores, L., Kantún-Moreno, N., Zwiers, L. H., Tzec-Simá, M., Peraza-Echeverría, S., ...

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Repositorio Institucional CICY

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