Título

Direct colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola

Autor

ROBERTO CARLOS VAZQUEZ EUAN

ROSA GRIJALVA ARANGO

BARTOLOME HUMBERTO CHI MANZANERO

MIGUEL ALONSO TZEC SIMA

IGNACIO RODRIGO ISLAS FLORES

CECILIA MONICA RODRIGUEZ GARCIA

LETICIA PERAZA ECHEVERRIA

ANDREW CHRISTOPHER JAMES

GILBERTO MANZO SANCHEZ

BLONDY BEATRIZ CANTO CANCHE

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

doi: DOI: 10.5897/AJB11.4044

Materias

SIGATOKA DISEASES - (AUTORES) BANANA’S MYCOSPHAERELLA - (AUTORES) MOLECULAR DIAGNOSIS - (AUTORES) DIRECT COLONY PCR - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

Sigatoka disease is the most important threat for banana production worldwide. Many species of Mycosphaerella have been described from banana but, to date, the three species Mycosphaerella fijiensis, M. musicola and M. eumusae are the only species found to be pathogenic to banana. Reliable identification by classical methods requires expertise because these fungi produce similar symptoms and they are morphologically similar. For studies of ecology, genetic diversity and epidemiology their differentiation is crucial. Several laboratories have developed molecular protocols to differentiate these fungi. Currently, a number of primers targeting ribosomal sequences, actin, tubulin and histone 3 genes are available for diagnosis of the Sigatoka complex. In the present work, we report a direct colony-polymerase chain reaction (DC-PCR) approach to rapidly distinguish M. fijiensis and M. musicola strains in multiplex PCR reactions. This is the most economical and the fastest procedure reported so far for diagnosis of these two Mycosphaerella species, which are distributed in banana-growing regions in the world; the DC-PCR technique was also found to be amenable for the identification of mating type of M. fijiensis isolates. This DC-PCR may also be applicable to prepare DNA templates for basic PCR-based analyses in other fungi.

Fecha de publicación

2012

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1047

Formato

application/pdf

Fuente

African Journal of Biotechnology, 11(33), 8172-8180, 2012

Idioma

Inglés

Relación

&

Canto-Canché, B. (2012). Direct colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola. African Journal of Biotechnology, 11(33), 8172-8180.

Sugerencia de citación

Vázquez-Euán, R., Grijalva-Arango, R., Chi-Manzanero, B., Tzec-Simá, M., Islas-Flores, I., Rodríguez-García, C., ...

Repositorio Orígen

Repositorio Institucional CICY

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