Título

Early Detection of White Spot Syndrome Virus (WSSV) in Isolated Hemocytes of Litopenaeus vannamei

Autor

ARIADNE HERNANDEZ PEREZ

Rossanna Rodríguez Canul

EDGAR TORRES IRINEO

JOSE FERNANDO MENDOZA CANO

DANIEL EDUARDO CORONADO MOLINA

JESUS ALEJANDRO ZAMORA BRISEÑO

JORGE HERNANDEZ LOPEZ

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de publicación

doi: DOI: 10.4236/cellbio.2017.61001

ISSN/ISSN: 2325-7792

URL/URL: http://www.scirp.org/journal/cellbio

Materias

WSSV, Litopenaeus vannamei , Ie1 Gene, Hemocytes, In Vitro Infection, GLS - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA ANIMAL (ZOOLOGÍA) - (CTI) VIROLOGÍA - (CTI)

Resumen o descripción

"To date, White Spot Syndrome (WSS) produced by the White Spot Syndrome Virus (WSSV) causes one of the most severe diseases infecting penaeid shrimps worldwide. Although a vast amount of studies has elucidated pathogenesis in live infection models, there is still little information about the interaction of WSSV infections using in vitro models in the whiteleg shrimp Litopenaeus vannamei (L. vannamei ) hemocytes. In this study, a WSSV infection kinetics was performed using total hemocytes isolated from healthy L. vannamei organisms and maintained in in vitro conditions using isotonic solution for shrimp (ISS). The infected experimental cells received ≈ 0,000 viral copies of WSSV. The viability of the hemocytes (control and infected group) was measured during the kinetics with trypan blue exclusion method and cells were maintained up to 6 hpi (post-infection) with non-significant differences of viability between both groups. WSSV replication was assessed using RTPCR at the RNA expression level of the early viral gene Ie1 and transcripts were detected as early as 30 min pi. Hemocytes from WSSV group showed disrupted integrity, degranulation and irregular shape. This study provides evidence of the capability of WSSV to infect and replicates in L. vannamei hemocytes using in vitro assays in short times as 30 min."

Editor

Scientific Research Publishing, Inc.

Fecha de publicación

2017

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/963

Formato

application/pdf

Fuente

CellBio

Idioma

Inglés

Sugerencia de citación

Hernández-Pérez, A. , Rodríguez-Canul, R. , Torres-Irineo, E. , Mendoza-Cano, F. , Coronado-Molina, D. , Zamora-Briseño, J. and Hernández-López, J. (2017) Early Detection of White Spot Syndrome Virus (WSSV) in Isolated Hemocytes of Litopenaeus vannamei. CellBio, 6, 1-12.

Repositorio Orígen

Repositorio Institucional CIBNOR

Descargas

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