Título

The Opuntia streptacantha OpsHSP18 Gene Confers Salt and Osmotic Stress Tolerance in Arabidopsis thaliana

Autor

SILVIA SALAS MUÑOZ

GRACIA ALICIA GOMEZ ANDURO

Pablo Delgado Sánchez

Margarita Rodríguez-Kessler

JUAN FRANCISCO JIMENEZ BREMONT

Nivel de Acceso

Acceso Abierto

Referencia de publicación

doi: DOI: doi.org/10.3390/ijms130810154

URL/URL: https://www.mdpi.com/1422-0067/13/8/10154

ISSN/ISSN: 16616596

Resumen o descripción

"Abiotic stress limits seed germination, plant growth, flowering and fruit quality, causing economic decrease. Small Heat Shock Proteins (sHSPs) are chaperons with roles in stress tolerance. Herein, we report the functional characterization of a cytosolic class CI sHSP (OpsHSP18) from Opuntia streptacantha during seed germination in Arabidopsis thaliana transgenic lines subjected to different stress and hormone treatments. The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT. On the other hand, the over-expression of the OpsHSP18 gene enhanced tolerance to salt (150 mM NaCl) and osmotic (274 mM mannitol) stress in Arabidopsis seedlings treated during 14 and 21 days, respectively. These plants showed increased survival rates (52.00 and 73.33%, respectively) with respect to the WT (18.75 and 53.75%, respectively). Thus, our results show that OpsHSP18 gene might have an important role in abiotic stress tolerance, in particular in seed germination and survival rate of Arabidopsis plants under unfavorable conditions."

Editor

Multidisciplinary Digital Publishing Institute

Fecha de publicación

2012

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Formato

application/pdf

Fuente

International Journal of Molecular Sciences

Idioma

Inglés

Sugerencia de citación

Salas-Muñoz, S.; Gómez-Anduro, G.; Delgado-Sánchez, P.; Rodríguez-Kessler, M.; Jiménez-Bremont, J.F. The Opuntia streptacantha OpsHSP18 Gene Confers Salt and Osmotic Stress Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2012, 13, 10154-10175. https://doi.org/10.3390/ijms130810154

Repositorio Orígen

Repositorio Institucional CIBNOR

Descargas

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