Advanced search


Knowledge area




Filter by:

Publication type

Authors

Issue Years

Publishers

Origin repository

Access Level

Language

Subject

Select the topics of your interest and receive the hottest publications in your email

3 results, page 1 of 1

Micronuclei and nuclear anomalies in Mexico’s indigenous population

BLANCA PATRICIA LAZALDE RAMOS (2017)

Objective. To determine the number of micronuclei and nuclear anomalies in Mexico’s indigenous population. Materials and methods. One hundred twenty indigenous individuals were evaluated, including thirty from the ethnicities Cora, Huichol, Tarahumara and Tepehuano. The number of micronuclei (MN) and any nuclear abnormality (NA) in oral mucosa cells, including cells with nuclear buds, binucleated cells, cells with karyolysis, karyorrhetic, condensed chromatin and pyknotic cells were determined for each participant. Results. Tepehuano and Tarahumaras showed the greatest damage to DNA. The Tepehuano group presented the highest number of MN and NA, this being a significant difference (p < 0.05) compared with the rest of the studied groups. This group also presented the highest herbicide exposure (46.7%). In relation to the smoking and drinking habits, these were more frequent in the Tarahumara group (33.3 and 50% respectively). Conclusion. The ethnic diversity, habits and customs may influence the DNA nuclear integrity in the Amerindian groups.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA micronuclei nuclear abnormalities indigenous population DNA

Ultraviolet-A Light Induces Micronucleated Erythrocytes in Newborn Rats

BLANCA PATRICIA LAZALDE RAMOS (2016)

Background: Ultraviolet-A (UV-A) light induce DNA damage by

creating pyrimidine dimers, or indirectly affects DNA by the formation

of reactive oxygen species. The objective was to determine DNA

damage by micronucleus test in neonatal rats exposed to UV-A

light.

Methods: Rat neonates were exposed to light from a LED

lamp (control group), to UV-C light 254 nm (control group to

desquamation skin) or UV-A light 365 nm and in one group the

dams were supplemented with folic acid (FA), to determine micro

nucleated erythrocytes (MNE) and micro nucleated polychromatic

erythrocytes (MNPCE) in peripheral blood of offspring.

Results: All the rat neonates exposed to UV-C lamp showed

desquamation skin, while for UV-A lamp no desquamation was

observed, and there was MNE differences in all sampling times

(P<0.02) and for MNPCE in 9 min group (P=0.001). No differences

between the groups with and without FA were observed.

Conclusion: Increased MNE frequencies without apparent damage

to the skin could be induced with UV-A light exposure. Under these

conditions, FA no protected against UV-A light exposure. This study

shows a manner to quantify the genotoxic effects of UV-A light in

peripheral blood erythrocytes of rat neonates.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA UV-A light Folic acid Micronucleated erythrocytes DNA damage Neonates

Micronucleated erythrocytes in newborns rats exposed to three different types of ultraviolet-A (UVA) lamps from commonly uses devices

BLANCA PATRICIA LAZALDE RAMOS (2016)

Exposure to ultraviolet-A (UVA) light can accidentally cause adverse effects in the skin and eyes. UVA induces DNA damage directly by creating pyrimidine dimers or by the formation of reactive oxygen species that can indirectly affect DNA integrity. UVA radiation is emitted by lamps from everyday devices. In adult rats, micronucleated erythrocytes (MNE) are removed from the circulation by the spleen. However, in newborn rats, MNE have been observed in peripheral blood erythrocytes. The objective of this study was to use micronucleus tests to evaluate the DNA damage caused in newborn rats exposed to UVA light from three different types of UVA lamps obtained from commonly used devices: counterfeit detectors, insecticide devices, and equipment used to harden resins for artificial nails. Rat neonates were exposed to UVA lamps for 20 min daily for 6 days. The neonates were sampled every third day, and the numbers of MNE and micronucleated polychromatic erythrocytes (MNPCE) in the peripheral blood were determined. The rat neonates exposed to the three types of UVA lamps showed increased numbers of MNE and MNPCE from 48 h to 144 h (P < 0.05 and P < 0.001 respectively). However, no relationship was observed between the number of MNE and the wattage of the lamps. In conclusion, under these conditions, UVA light exposure induced an increase in MNE without causing any apparent damage to the skin.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA Ultraviolet light Radiation DNA damage Micronuclei Erythrocytes