Development of a next generation SNP genotyping array for wheat

Simon Griffiths Susanne Dreisigacker Alison Bentley Gina Brown-Guedira (2023)

Article

Axiom Array High Throughput Genotyping Hexaploid Wheat Copy Number Variation Analysis CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SINGLE NUCLEOTIDE POLYMORPHISMS WHEAT GENOTYPING GENETIC DIVERSITY (AS RESOURCE)

Fabrication of PVDF/PMMA Polymer for Sustainable Energy Harvesting .

JOSE RAYMUNDO LEPPE NEREY FERNANDO ZENAIDO SIERRA ESPINOSA MIGUEL ANGEL BASURTO PENSADO JOSE ALFREDO RODRIGUEZ RAMIREZ (2023)

The synthesis of blends that combine properties of two or more polymeric materials is increasingly investigated due to the versatility of the synthesis and its growing potential for many applications, including sustainability. Their characteristics are defined mainly by the synthesis conditions. Therefore, this paper details the synthesis process of easy-to-handle films using mixing method. The procedures and drawbacks found during the preparation of composite films are described. Polymeric compounds formed by the mixture of polyvinylidene fluoride (PVDF) and polymethyl methacrylate (PMMA) are addressed, varying the concentration, and evaluating their impact on the piezoelectric capacity. Films were formed through the spin-coating technique and characterized by optical and holographic microscopes. The results showed that composites with a concentration of 50 wt.% or larger of PVDF in the blend acquire a morphology with a granular appearance, however at lower concentrations they present a homogeneous morphology similar to that of PMMA. A homogeneous distribution of PVDF in the PMMA stands out. However, excessive contents of PMMA are associated to peaks and non-uniformities detected like multicolored regions by digital holography. Controlled strength-strain laboratory tests allowed to evaluate the film blends performance. The results indicate noticeable improvements in voltage output for a composition 70wt% PVDF and 30 wt% PMMA.

Article

INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS Polymer blends, Power generation, Energy harvesting, Piezoelectricity,

Complex traits and candidate genes: estimation of genetic variance components across multiple genetic architectures

GIOVANNY COVARRUBIAS-PAZARAN Hans-Peter Piepho (2023)

Article

Average Semivariance Linear Mixed Model Variance Component Estimation Polygenic Inheritance Oligogenic Inheritance Mendelian Inheritance CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MENDELISM GENETIC VARIANCE GENOME-WIDE ASSOCIATION STUDIES PHENOTYPES CHROMOSOME MAPPING

Molecular characterization of doubled haploid lines derived from different cycles of the Iowa Stiff Stalk Synthetic (BSSS) maize population

Jode Edwards Sarah Hearne (2023)

Article

Homozygous Lines CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GENETIC DIVERSITY (AS RESOURCE) GENETIC RESOURCES ZEA MAYS GENOTYPING DOUBLED HAPLOIDS SINGLE NUCLEOTIDE POLYMORPHISMS

Evaluación de la cinética de liberación de compuestos hidrofílicos y lipofílicos a partir de nanopartículas híbridas polímero-lípido

Evaluation of the release kinetics of hydrophilic and lipophilic compounds from polymer-lipid hybrid nanoparticles

Juan Pablo Carmona Almazán (2023)

En el tratamiento de enfermedades, la administración de dosis múltiples es una estrategia común para mantener la concentración de los fármacos dentro de un margen terapéutico. Sin embargo, la adherencia de los pacientes a este tipo de tratamiento puede ser un desafío, llevando a una administración irregular de dosis. Una alternativa utilizada para abordar este reto son las nanopartículas híbridas polímero/lípido(NPPLs), las cuales, con menos administraciones, tienen el potencial de alcanzar la dosis necesaria en el tratamiento, posibilitando entonces el incremento del apego al tratamiento. En nuestro proyecto, se llevó a cabo la síntesis de nanopartículas de ácido poli láctico-co-glicólico (PLGA) recubiertas de lecitina de soya, por medio de técnicas de nanoprecipitación y autoensamblaje. Además, integramos estas nanopartículas en una matriz polimérica a base de aerogeles de gelatina de manera que estuvieran distribuidas de manera homogénea y concentrada. Nuestro enfoque central radica en entender la cinética de liberación de un compuesto hidrofílico (ácido gálico) y uno lipofílico (quercetina) a partir de este sistema. Logramos sintetizar nanopartículas con un diámetro hidrodinámico de 100 ± 15 nm, 153 ± 33 y149±21 nm, en el caso de las nanopartículas vacías y cargadas con ácido gálico y cargadas con quercetina, respectivamente. La eficiencia de encapsulación del ácido gálico fue del 90 ± 5 % y de la quercetina fue del 70 ± 10 %. Los resultados que obtuvimos muestran que el ácido gálico sigue una cinética del modelo de Korsmeyer-Peppas, con un valor de n = 1.01 y la quercetina una cinética de primer orden. Dado que los compuestos encapsulados tuvieron una liberación más lenta con respecto a los compuestos libres en los aerogeles de gelatina, nuestro trabajo indica que el encapsulamiento en NPPLs de un compuesto bioactivo, independientemente de su naturaleza química, puede ayudar a retrasar su liberación y reducir el número de dosis administradas, en consecuencia, esto pudiera contribuir a incrementar el apego de un paciente al tratamiento.

In the treatment of diseases, the administration of multiple doses is a common strategy to maintain drug concentrations within a therapeutic range. However, patient adherence to this type of treatment can be challenging, resulting in irregular dosing. An alternative approach used to address this challenge involves polymer/lipid hybrid nanoparticles (NPPLs), which have the potential to achieve the necessary drug dose with fewer administrations, thereby increasing treatment adherence. In our project, we synthesized poly(lactic-co-glycolic acid) (PLGA) nanoparticles coated with soy lecithin using nanoprecipitation and self-assembly techniques. These nanoparticles were then integrated into a polymer matrix based on gelatin aerogels to ensure homogeneous and concentrated distribution. Our main focus was to understand the release kinetics of a hydrophilic compound (gallic acid) and a lipophilic one (quercetin) from this system. We successfully synthesized nanoparticles with a hydrodynamic diameter of 100 ± 15 nm, 153 ± 33 nm, and 149 ± 21 nm for empty nanoparticles, gallic acid-loaded, and quercetin-loaded nanoparticles, respectively. The encapsulation efficiency was 90 ± 5 % for gallic acid and 70 ± 10 % for quercetin. The results we obtained indicate that gallic acid follows Korsmeyer-Peppas kinetics with a value of n = 1.01, while quercetin exhibits first-order kinetics. Since the encapsulated compounds showed slower release compared to free compounds in gelatin aerogels, our work suggests that encapsulation in NPPLs with a bioactive compound, regardless of its chemical nature, can help delay its release and reduce the number of doses administered. Consequently, this could contribute to improve patient treatment adherence.

Master thesis

nanopartículas híbridas, cinética de liberación, sistemas poliméricos, PLGA/lecitina, compuestos hidrofílicos y lipofílicos hybrid nanoparticles, release kinetics, polymeric systems, PLGA/lecithin, hydrophilic and lipophilic compounds INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS INGENIERÍA Y TECNOLOGÍA QUÍMICAS ANÁLISIS DE POLÍMEROS ANÁLISIS DE POLÍMEROS

QTL cluster analysis and marker development for kernel traits based on DArT markers in spring bread wheat (Triticum aestivum L.)

CAIXIA LAN Ravi Singh (2023)

Article

QTL mapping Kernel-Related Traits Putative Candidate Gene KASP Markers CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA KERNELS QUANTITATIVE TRAIT LOCI MAPPING TRITICUM AESTIVUM SINGLE NUCLEOTIDE POLYMORPHISM

Editorial: Genome wide association studies and genomic selection for crop improvement in the era of Big Data

Alison Bentley Charles Chen Nunzio D'Agostino (2022)

Article

Allele Mining High-Throughput Phenotyping Genomic Estimated Breeding Value CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CROP IMPROVEMENT DNA CHROMOSOME MAPPING GENETIC LINKAGE GENOMES GENOTYPING GERMINATION HEAT STRESS QUALITY CONTROL SINGLE NUCLEOTIDE POLYMORPHISM TRITICUM AESTIVUM GENETIC DIVERSITY (AS RESOURCE) HIGH-THROUGHPUT SEQUENCING

Field deployable Reverse Transcriptase–Recombinase Polymerase Amplification (RT-RPA) for detection of Maize chlorotic mottle virus (MCMV)

Margaret Redinbaugh Suresh L.M. (2022)

Article

Maize Lethal Necrosis Diagnostics Recombinase Polymerase Amplification CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE MAIZE CHLOROTIC MOTTLE VIRUS DISEASES DETECTION

Identification of genetic loci for grain yield-related traits in the wheat population Zhongmai 578/Jimai 22

Jingyang Tong Ming Li xianchun xia Zhonghu He Yong Zhang (2023)

Article

Grain Yield KASP Marker QTL Mapping SNP Chip CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GRAIN YIELDS QUANTITATIVE TRAIT LOCI MAPPING SINGLE NUCLEOTIDE POLYMORPHISMS WHEAT BREEDING

SNP discovery using BSR-Seq approach for spot blotch resistance in wheat (Triticum aestivum L.), an essential crop for food security

Vinod Mishra Ramesh Chand UTTAM KUMAR Apurba Chowdhury arun joshi (2022)

Article

Spot Blotch Recombinant Inbred Lines Bulk Segregant Analysis CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT FOOD SECURITY SINGLE NUCLEOTIDE POLYMORPHISM DISEASE RESISTANCE