Author: VICTOR ARTURO GONZALEZ HERNANDEZ
Con el objeto de ubicar anatómicamente los flavonoles en guayaba (Psidium guajava L.), se hicieron cortes en fresco, y se observo al microscopio de fluorescencia con un filtro de 520 nm de excitación y 490 nm de emisión. La fluorescencia fue positiva en la epidermis adaxial, abaxial y glándulas en hoja madura, en hoja joven solo presentó en la epidermis adaxial, en el fruto fue positivo en la epidermis y en espacios esquizógenos, y en tallo en espacios esquizógenos, en protofloema y tricomas
This study is focused on finding (Psidium guajava L.) guava's flavonols. Fresh cuts were obtained in order to observe the orange fluorescence, using a microscope with 520 nm excitation and 490 nm emission. Fluorescence was positive in adaxial epidermis, abaxial and glands of mature leaves. In young leaves, fluorescence was only found in adaxial epidermis. In fruit, fluorescence was positive in epidermis and schizogenetic spaces. In stem, fluorescence was found in schizogenetic spaces, and protophloem, and trichomes
The study was conducted in the village of Filo de Caballos, municipality of Leonardo Bravo, Guerrero, Mexico, from November 2011 to October 2012, in an orchard of Hass avocado, 5 years old. The objective was to evaluate the treatment of chemical and organic nutrition of avocado trees; fertilizers used were: 50 kg tree of cattle dung (manure); 200-100-200 kg ha-1 of N-P-K (edaphic) and 30-10-15.30 kg ha-1 of Ca-Mg-B (foliar). We found that in buds, treatment manure + edaphic + foliar (41 shoots/branch) was better than treatments manure + edaphic (19 shoots/branch), leaf (9 shoots/branch) and the control (21 shoots/branch). And with 17 fruits, manure + treatment + foliar edaphic significantly exceeded the edaphic + foliar treatments (6 fruits), leaf (1 fruit) and the control (3 fruits). The manure + foliar treatment had the highest length (16 cm) of the main shoot, beating the treatment of manure + edaphic (7 cm) and leaf (9 cm). The edaphic + foliar treatment (75 cm2 ) had higher leaf area than control (65 cm2 ) and had the highest fruit weight 2.62 kg with 10 fruits, which was significantly higher than the control (1.51 kg 10 fruits).
VICTOR ARTURO GONZALEZ HERNANDEZ MARIA GRICELDA VAZQUEZ CARRILLO AMALIO SANTACRUZ VARELA ADRIANA DELGADO ALVARADO Ma. Claudia Castañeda Saucedo LEOBIGILDO CORDOVA TELLEZ Ernesto Tapia Campos GABINO GARCIA DE LOS SANTOS (2012)
"Changes in the concentration of sucrose, glucose, fructose and starch, in leaves, seeds and pods of Phaseolus vulgaris L. cv "Otomí" of determinate growth habit, were evaluated under both drought stress and non stress (irrigated) conditions. Plants were submitted to drought stress during the flowering, pod formation and seed filling stages. The stress increased the concentrations of glucose from 18 up to 196% and of fructose from 20 up to 333% in mature leaves, likely due to starch hydrolysis, while showing starch reductions from 17 up to 46% and sucrose reductions from 41 up to 70%. These changes in leaves promoted the sugar exports toward pods and seeds, since their sucrose concentration increased. In the reproduc- tive organs, the concentration of glucose and fructose of stressed plants increased 136 and 138% in pods and 83 and 73% in seeds, respectively. Contrary to the changes registered in leaves, in repro- ductive tissues the sucrose increased up to 238% in pods and up to 148% in seeds, while the starch became reduced down to 37% in pods and to 21% in seeds. The increase of hexoses in pods and seeds might be explained by in situ starch hydrolysis, whereas the increase in sucrose is probably due to an enhanced synthesis of this sugar for a higher osmotic adjustment"
Ma. Claudia Castañeda Saucedo LEOBIGILDO CORDOVA TELLEZ Ernesto Tapia Campos ADRIANA DELGADO ALVARADO VICTOR ARTURO GONZALEZ HERNANDEZ AMALIO SANTACRUZ VARELA HERMINIA DE JESUS LOZA TAVERA GABINO GARCIA DE LOS SANTOS MARTIN PEDRO VARGAS SUAREZ (2013)
"Drought is a major constraint for common bean (Phaseolus vulgaris L.) production in México. Dehydrins are constitutive or stressinduced proteins related with a protective role of membranes and macromolecules against denaturation, thus preventing loss of their function. In this work, seed production and patterns of dehydrins accumulation in leaves and pods were evaluated in common bean cv. ‘Otomí’ subjected to drought, as compared with well-irrigated plants. Drought applied at pod formation and seed filling (SF) reduced yield up to 57 %. An antibody against a consensus sequence present in most dehydrins allowed for dehydrin identification. Two dehydrins of 82 and 73 kDA turned up both in leaves and pods throughout all the evaluated conditions. Presumably, both dehydrins are constitutive in the ‘Otomí’ cultivar. These dehydrins showed higher expression than controls in leaves after 6 d of drought at seedling and SF stages, and in pods 6 d after drought had started at SF. Increased expression might provide better protection during early stages of seedling and seed development. Increments on 63, 36 and 22 kDa dehydrin expression in pods at late SF might coincide with plant developmental programs, which prepare seed for desiccation. Dehydrins of 158, 54, 46, and 41 kDa were detected in pods 10 d after floral opening as a transient response to drought stress in SF. These results indicate dehydrins are relevant during plant development, as well as during drought stress."