Author: TOMAS GONZALEZ ESTRADA

Establecimiento de un Jardín Botánico en Mérida, Yucatán

ROGER ARMANDO ANTONIO ORELLANA LANZA TOMAS AUGUSTO GONZALEZ ESTRADA VERONICA FRANCO TORIZ Lina Ojeda Revah (1986)

México se caracteriza por presentar una rica vegetación, producida por la interacción físico biótica y las actividades humanas. En su territorio, convergen elementos de la Flora Neotropical, Neártica y Antillana. La península de Yucatán, es una importante región en la que el desarrollo moderno ha impactado su riqueza florística original. Esta es la razón por la cual surgió la necesidad de plantear la existencia de un Jardín Botánico Regional, donde se represente lo más importante de la región y se tomen medidas para la preservación del germoplasma nativo con objetos de apoyar la investigación, experimentar; y difundir la importancia de las plantas de la región. El Jardín Botánico Regional del CICY, se estableció al norte de la ciudad de Mérida, en un clima cálido subhúmedo, con suelos pedregosos de una antigua plantación de henequén (Agave fourcroydes) que ha regenerado a vegetación secundaria de Selva Baja decidua, donde se representan aproximadamente 200 especies. En esta área se disoñó el Jardín Botánico con cinco secciones: Arboretum, área de colecciones especiales, ornamental, de reserva y experimental. En este, actualmente se tienen registrados cerca de 4000 especímenes y se realizan experiencias tendientes a la preservación del plasma germinal nativo, con el que se pretende incidir sobre las medidas de conservación regionales.

Article

JARDINES BOTANICOS BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Endogenous GUS-like activity in Capsicum chinense Jacq.

LAURA YESENIA SOLÍS RAMOS TOMAS AUGUSTO GONZALEZ ESTRADA ANTONIO ANDRADE TORRES GREGORIO DEL CARMEN GODOY HERNANDEZ ENRIQUE CASTAÑO DE LA SERNA (2010)

The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels of intensity. Histochemical screening showed that endogenous GUS-like activitydecreased, or reduced significantly, in almost all tissues with exception of stament, when phosphate buffer was adjusted to pH 8. Subsequently, C. chinense zygotic embryo explants were transient transformed with Agrobacterium tumefaciens LBA4404 (pCAMBIA2301) and plantlets regenerated were histochemically stained in phosphate buffer pH 8. Observations of incubated tissues of C. chinense regenerants showed blue staining, suggesting expression of uidA. Incubated tissues of non-transformed regenerants did not show blue staining in phosphate buffer pH 8. The results show that for transformation experiments of C. chinense with uidA gene, pH 8 is recommended for histochemical staining.

Article

ß-GLUCURONIDASE AGROBACTERIUM TUMEFACIENS CAPSICUM CHINENSE GENE UIDA BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Characterization of a Capsicum chinense seed peptide fraction with broad antibacterial activity

LIGIA GUADALUPE BRITO ARGAEZ FERNANDO FEDER MOGUEL SALAZAR FERNANDO ZAMUDIO ZUÑIGA TOMAS GONZALEZ ESTRADA IGNACIO RODRIGO ISLAS FLORES (2009)

Habanero chili pepper (Capsicum chinense) is widely consumed as a fresh vegetable, although its extremely high capsaicin content has led to other uses (e.g., medicine and self-defense). Recently described antimicrobial peptides from C. annuum were very efficient in inhibiting growth in human and plant pathogenic bacteria and fungi. In order to explore the potential use of Capsicum chinenseseeds as a source of antimicrobial peptides, in the present study a peptide fraction from C. chinense pepper seeds, denominated G10P1, was enriched, partially purified and its antimicrobial activitytested against the plant and human pathogens Xanthomonas campestris, Pseudomonas syringae, Pseudomonas aeruginosa, Erwinia carotovora, Agrobacterium sp., Shigella flexnerii, Escherichia coli, Staphyllococcus aureus and Bacillus subtilis. The minimum inhibitory concentration of the G10P1 peptide fraction against X. campestris was 12.5 μg mL-1. Electrophoresis of the G10P1 in a denaturant 15% polyacrylamide gel showed it to be composed of ~7.57 and ~5.6 kDa polypeptides, both associated with an area of strong antibacterial activity. The sequencing of 18 amino acids from the N-terminal of the ~7.57 peptides and 12 from the ~5.6 kDa peptides showed no clear association with previously described antimicrobial peptides. However, the ~5.6 kDa peptides were related to the NAC and WRKY transcription factors, both involved in direct regulation of the plant defense response against pathogen attack and the ~7.57 kDa peptides had low homology with a 3-oxo-[acyl-carrier-protein] synthase from Capsicum chinense.

Article

ANTIMICROBIAL PEPTIDES CAPSICUM CHINENSE COLUMN CHROMATOGRAPHY HABANERO PEPPER PLANT ANTIMICROBIAL PEPTIDES BIOLOGÍA Y QUÍMICA

Efficient plant regeneration from leaf explants of Solanum americanum

INGRID AILEEN O'CONNOR SANCHEZ ANGEL VIRGILIO DOMINGUEZ MAY MIGUEL ANGEL KEB LLANES TOMAS AUGUSTO GONZALEZ ESTRADA YURI JORGE JESUS PEÑA RAMIREZ (2010)

A very efficient system for direct plant regeneration from in vitro-derived leaf explants of Solanum americanum was developed. S. americanum is a tropical plant with important medical properties. The in vitro procedure that was established consists of (i) induction of shoots from leaf tissue, (ii) elongation of shoots, and (iii) rooting of plantlets. The induction of shoots was achieved on Murashige and Skoog solid medium supplemented with different combinations of zeatin riboside and 1-naphthalene acetic acid or 6-benzylaminopurine and 1-naphthalene acetic acid. The best combination for plant regeneration was MS with 5.7 μM zeatin riboside and 0.11 μM 1-naphthalene acetic acid. In the second step, the shoot clumps were transferred to MS basal medium without plant growth regulators, resulting in the differentiation of most of the shoot initials into well developed shoots. In the third stage, plantlets were efficiently rooted on half-strength MS basal medium supplemented with 58.5 mM sucrose. The rooted plants were established in soil with a 100% success rate. This system can be useful to perform further experiments to obtain transgenic plants of this species as well as for other biotechnological approaches. © 2010 Academic Journals.

Article

1-NAPHTHALENE ACETIC ACID AMERICAN NIGHTSHADE IN VITRO CULTURE MEDICINAL PLANT SPECIES ORGANOGENESIS SHOOTS REGENERATION ZEATIN RIBOSIDE BIOLOGÍA Y QUÍMICA

Efficient plant regeneration from leaf explants of Solanum americanum

INGRID AILEEN O'CONNOR SANCHEZ ANGEL VIRGILIO DOMINGUEZ MAY MIGUEL ANGEL KEB LLANES TOMAS AUGUSTO GONZALEZ ESTRADA YURI JORGE JESUS PEÑA RAMIREZ (2010)

A very efficient system for direct plant regeneration from in vitro-derived leaf explants of Solanum americanum was developed. S. americanum is a tropical plant with important medical properties. The in vitro procedure that was established consists of (i) induction of shoots from leaf tissue, (ii) elongation of shoots, and (iii) rooting of plantlets. The induction of shoots was achieved on Murashige and Skoog solid medium supplemented with different combinations of zeatin riboside and 1-naphthalene acetic acid or 6-benzylaminopurine and 1-naphthalene acetic acid. The best combination for plant regeneration was MS with 5.7 μM zeatin riboside and 0.11 μM 1-naphthalene acetic acid. In the second step, the shoot clumps were transferred to MS basal medium without plant growth regulators, resulting in the differentiation of most of the shoot initials into well developed shoots. In the third stage, plantlets were efficiently rooted on half-strength MS basal medium supplemented with 58.5 mM sucrose. The rooted plants were established in soil with a 100% success rate. This system can be useful to perform further experiments to obtain transgenic plants of this species as well as for other biotechnological approaches. © 2010 Academic Journals.

Article

1-NAPHTHALENE ACETIC ACID AMERICAN NIGHTSHADE IN VITRO CULTURE MEDICINAL PLANT SPECIES ORGANOGENESIS SHOOTS REGENERATION ZEATIN RIBOSIDE BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA