Author: BLANCA PATRICIA LAZALDE RAMOS

Paternal Determinants in Preeclampsia

BLANCA PATRICIA LAZALDE RAMOS (2019)

Preeclampsia is a condition associated with high rates of maternal-fetal morbidity and mortality. It usually occurs in 3–10% of nulliparous women and 18% of previously affected women. Different lines of evidence have demonstrated the role of the father in the onset of preeclampsia. The placenta is the cornerstone of preeclampsia and poses important paternal genetic determinants; in fact, the existence of a “paternal antigen” has been proposed. Nulliparity is a well-known risk factor. Change of partner to a woman without history of preeclampsia increases the risk; however, this change decreases in women with history of the condition. High interval between pregnancies, short sexual intercourse before pregnancy, and conception by intracytoplasmic sperm injection suggest a limited exposure to the so-called paternal antigen. A man who was born from a mother with preeclampsia also increases the risk to his partner. Not only maternal but also paternal obesity is a risk factor for preeclampsia. Fetal HLA-G variants from the father increased the immune incompatibility with the mother and are also significantly associated with preeclampsia in multigravida pregnancies. An analysis of a group of Swedish pregnant women showed that the risk for preeclampsia is attributable to paternal factors in 13% of cases, which could be related to genetic interactions with maternal genetic factors. This review aimed to evaluate the evidences of the father’s contribution to the onset of preeclampsia and determine the importance of including them in future studies.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA Preeclampsia paternal genetic determinants immune incompatibility

Ultraviolet-A Light Induces Micronucleated Erythrocytes in Newborn Rats

BLANCA PATRICIA LAZALDE RAMOS (2016)

Background: Ultraviolet-A (UV-A) light induce DNA damage by

creating pyrimidine dimers, or indirectly affects DNA by the formation

of reactive oxygen species. The objective was to determine DNA

damage by micronucleus test in neonatal rats exposed to UV-A

light.

Methods: Rat neonates were exposed to light from a LED

lamp (control group), to UV-C light 254 nm (control group to

desquamation skin) or UV-A light 365 nm and in one group the

dams were supplemented with folic acid (FA), to determine micro

nucleated erythrocytes (MNE) and micro nucleated polychromatic

erythrocytes (MNPCE) in peripheral blood of offspring.

Results: All the rat neonates exposed to UV-C lamp showed

desquamation skin, while for UV-A lamp no desquamation was

observed, and there was MNE differences in all sampling times

(P<0.02) and for MNPCE in 9 min group (P=0.001). No differences

between the groups with and without FA were observed.

Conclusion: Increased MNE frequencies without apparent damage

to the skin could be induced with UV-A light exposure. Under these

conditions, FA no protected against UV-A light exposure. This study

shows a manner to quantify the genotoxic effects of UV-A light in

peripheral blood erythrocytes of rat neonates.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA UV-A light Folic acid Micronucleated erythrocytes DNA damage Neonates

Genome Damage in Rats after Transplacental Exposure to Jatropha dioica Root Extract

BLANCA PATRICIA LAZALDE RAMOS (2019)

Jatropha dioica is traditionally used owing to its antiviral, antifungal, and antimicrobial properties. But, toxicological information regarding J. dioica root total extract is currently limited. The aim of this work was to evaluate in a rat model, the transplacental genotoxicity effect of J. dioica aqueous root total extract. Three different J. dioica aqueous root total extract doses (60, 100, and 300 mg/kg) were administered orally to Wistar rats during 5 days through the pregnancy term (16–21 days). Pregnant rats were sampled every 24 h during the last 6 days of gestation, and pubs were sampled at birth. Genome damage in dams and their newborn pups transplacentally exposed to J. dioica was evaluated by in vivo micronuclei assay. We evaluated the frequency of micronucleated erythrocytes (MNE), micronucleated polychromatic erythrocytes (MNPCE), and polychromatic erythrocytes (PCE) in peripheral blood samples from pups and MNPCE and PCE in pregnant rats. No genotoxic effect was observed after oral administration of the three different doses of aqueous root total extract of J. dioica in pregnant or in their newborn pubs, after transplacental exposure. A significant decrease in PCE frequency was noted in samples from pubs of rats treated with the highest dose of J. dioica extract. The aqueous total root extract of J. dioica at the highest dose tested in our research do have cytotoxic effect in pups transplacentally exposed to this plant extract. Moreover, neither a genotoxic nor a cytotoxic effect was observed in pregnant rats. In the present work, there was no evidence of genome damage in the rat model after transplacental exposure to J. dioica aqueous root total extract.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA Jatropha dioica micronucleated erythrocytes (MNE) genome damage

Patients with advanced oral squamous cell carcinoma have high levels of soluble E-cadherin in the saliva

BLANCA PATRICIA LAZALDE RAMOS (2017)

Background: The objective of this study was to assess the potential clinical value of the concentration of soluble salivary E-cadherin (sE-cadherin) compared with the clinical value of the presence of membranous E-cadherin (mE-cadherin) in oral squamous cell carcinoma tumor tissues. Material and Methods: Data regarding patient demographics, clinical stage, saliva and tumor tissue samples were collected. The saliva was analyzed for sE-cadherin protein levels and was compared to the mE-cadherin immu-nohistochemical expression levels in tumor tissues, which were assessed via the HercepTest® method. Patients without cancer were included in the study as a control group for comparisons of the sE-cadherin levels. Results: sE-cadherin levels in the saliva of patients without cancer were lower than those in patients with cancer, and the difference was statistically significant (p=0.031). Low mE-cadherin expression was statistically signifi-cantly associated with lymph node positivity (p=0.015) and advanced clinical stage (p=0.001). The inverse rela-tionship between mE-cadherin and sE-cadherin was significant in terms of lymph node positivity (p=0.014) and advanced clinical stage (p=0.037). Conclusions: The results suggest that sE-cadherin levels are significantly increased in patients with oral cancer and that its low expression within the membrane as well as the progression of the disease appear to be inversely associ-ated with levels of sE-cadherin in the saliva.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA E-cadher.in saliva oral cancer

Influence of CYP1A1*2C on High Triglyceride Levels in Female Mexican Indigenous Tarahumaras

BLANCA PATRICIA LAZALDE RAMOS (2014)

Background and Aims

High triglyceride levels are closely related to cardiovascular disease. Its development lays on age, diet, physical activity, ethnicity and genetic factors. Among the last, the CYP1A1*2C allele has an influence on the metabolism of cholesterol and other fatty acids. We undertook this study to determine the frequency of CYP1A1*2C and its association with triglyceride levels in Mexican indigenous Tarahumaras and Tepehuanos.

Methods

Anthropometric and biochemical data were recorded. Genotyping of CYP1A1*2C by RT-PCR was done in 110 Tepehuano, 69 Tarahumara and 64 Mestizo.

Results

Significant differences in age, waist diameter, BMI, creatinine, glucose, cholesterol, triglycerides, HDL and VLDL measurements were found between Tarahumaras and Tepehuanos (p <0.05). Additionally, Tarahumara women showed the highest values of waist diameter, BMI and triglycerides (p <0.05). It was found that Tarahumaras showed a significant association between high triglyceride levels and CYP1A1*2C allele (OR = 2.57; 95% CI 1.12–5.88, p = 0.024) under a recessive inheritance model. However, the Tepehuano group showed a significant protective association between normal triglyceride levels and CYP1A1*2C polymorphism (OR = 0.28; 95% CI 0.10–0.80, p = 0.015) following a dominant inheritance model. The same pattern was observed after analysis with females of both ethnicities.

Conclusion

A significant association between CYP1A1*2C and high triglyceride levels in Amerindian Tarahumaras from Chihuahua has been found; this allele was significantly associated with normal triglyceride levels in Tepehuanos from Durango, Mexico. Further studies are needed to elucidate the genetic role of CYP1A1 in cardiovascular disease susceptibility.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA High triglyceride levels Tarahumaras Tepehuanos CYP1A1

Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases

BLANCA PATRICIA LAZALDE RAMOS (2014)

Background and objective

Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long-term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and DNA damage. The aim of this study was to evaluate DNA and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.

Material and methods

Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (n = 58), subjects with aggressive periodontitis (AgP) (n = 42) and a control group (n = 60). DNA damage was determined by counting micronuclei (MN) and nuclear abnormalities (NAs) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8-hydroxy-2'-deoxyguanosine (8-OHdG) in whole saliva.

Results

Subjects with CP or AgP presented significantly more ( p < 0.05) MN and NAs and higher levels of 8-OHdG ( p < 0.05) compared with the control group.

Conclusion

Our results indicate that subjects with periodontitis (CP or AgP) exhibited an increase in the frequency of MN, NAs and 8-OHdG, which is directly related to DNA damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and MN.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA Periodontal disease chronic bacterial infection Buccal mucosa cells

Micronucleated erythrocytes in newborns rats exposed to three different types of ultraviolet-A (UVA) lamps from commonly uses devices

BLANCA PATRICIA LAZALDE RAMOS (2016)

Exposure to ultraviolet-A (UVA) light can accidentally cause adverse effects in the skin and eyes. UVA induces DNA damage directly by creating pyrimidine dimers or by the formation of reactive oxygen species that can indirectly affect DNA integrity. UVA radiation is emitted by lamps from everyday devices. In adult rats, micronucleated erythrocytes (MNE) are removed from the circulation by the spleen. However, in newborn rats, MNE have been observed in peripheral blood erythrocytes. The objective of this study was to use micronucleus tests to evaluate the DNA damage caused in newborn rats exposed to UVA light from three different types of UVA lamps obtained from commonly used devices: counterfeit detectors, insecticide devices, and equipment used to harden resins for artificial nails. Rat neonates were exposed to UVA lamps for 20 min daily for 6 days. The neonates were sampled every third day, and the numbers of MNE and micronucleated polychromatic erythrocytes (MNPCE) in the peripheral blood were determined. The rat neonates exposed to the three types of UVA lamps showed increased numbers of MNE and MNPCE from 48 h to 144 h (P < 0.05 and P < 0.001 respectively). However, no relationship was observed between the number of MNE and the wattage of the lamps. In conclusion, under these conditions, UVA light exposure induced an increase in MNE without causing any apparent damage to the skin.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA Ultraviolet light Radiation DNA damage Micronuclei Erythrocytes

CYP2D6 gene polymorphisms and predicted phenotypes in eight indigenous groups from northwestern Mexico

BLANCA PATRICIA LAZALDE RAMOS (2014)

Aim: Polymorphisms in CYP2D6 impact the interindividual and interethnic variability of drug efficiency; therefore, we determined the CYP2D6 allele distribution in eight Amerindian groups from northwestern Mexico and compared them with the frequencies in Mexican Mestizos. Materials & methods: A total of 508 Amerindians were studied. Genotyping of CYP2D6 5 and multiplication alleles was performed by long-range PCR, while CYP2D62,3,4,6,10,17,29,35,41 and copy number were evaluated by real-time PCR. Results: The most frequent alleles were CYP2D62 (0.05-0.28), CYP2D64 (0.003-0.21) and multiplications (0.043-0.107). CYP2D65,6, * 10 and41 were not observed in the majority of Amerindians, and CYP2D63,17,35 and29 were not detected. The poor metabolizer genotype (4/5) was lower (0.2%) in Amerindians than in Mestizos (5%); conversely, the ultrarapid metabolizer genotype was higher (12.6%) in indigenous groups than in Mestizos (7%). Conclusion: Our data show a lower frequency of CYP2D6 inactive alleles and a higher frequency of duplication/multiplication of CYP2D6 active alleles in indigenous populations that in Mestizos.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA drug efficiency Mexican Mestizos CYP2D6

CYP450 Genotype/Phenotype Concordance in Mexican Amerindian Indigenous Populations–Where to from Here for Global Precision Medicine?

BLANCA PATRICIA LAZALDE RAMOS (2017)

Global precision medicine demands characterization of drug metabolism and phenotype variation in diverse populations, including the indigenous societies. A related question is the extent to which CYP450 drug metabolizing enzyme genotype and phenotype data are concordant and whether they can be used interchangeably. These issues are increasingly debated as precision medicine continues to expand as a popular research topic worldwide. We report here the first study in clinically relevant CYP450 drug metabolism phenotypes and genotypes in Mexican Amerindian indigenous subjects. In a large sample of 450 unrelated and medication free Mexican Amerindian indigenous healthy persons from four Mexican states (Chihuahua, Durango, Nayarit, and Sonora), we performed multiplexed phenotyping for the CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 drug metabolizing enzymes using the CEIBA cocktail and genotyped the same pathways for functional polymorphic variation. Remarkable interindividual variability was found for the actual drug metabolizing capacity of all the enzymes analyzed, and, more specifically, the metabolic ratios calculated were significantly different across individuals with different number of active alleles for CYP2C9, CYP2C19, and CYP2D6. The drug metabolizing capacity "predicted" from the genotype determined was not in accordance with the actual capacity "measured" by phenotyping in several individuals for CYP2C9, CYP2C19, and CYP2D6. Consequently, a more extensive genotyping of the main CYP enzymes, including rare variants, together with the analysis of the actual drug metabolizing capacity using an appropriate phenotyping approach will add valuable information for accurate drug metabolism studies, especially useful in understudied populations such as Mexican Amerindians. In sum, this study demonstrates that current personalized medicine strategies based on "predicted" phenotype from genotyping of alleles with high frequency in European populations are not adequate for Mestizos and Native American populations.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA biomarkers developing world omics drug metabolism variation global personalized medicine systems diagnostics

A shortened treatment with rosemary tea (rosmarinus officinalis) instead of glucose in patients with diabetes mellitus type 2 (TSD)

BLANCA PATRICIA LAZALDE RAMOS (2019)

Background

Rosemary leaves powder has been reported to reduce in a dose-dependent manner, glucose levels, lipid profile and lipid peroxidation in humans. However, patients should ingest high doses of powder contained in capsules. This formulation constitutes the intake of 10 capsules per day, so the active metabolite must first, be released and then absorbed (for which, rosemary leaf powder must be mixed with gastric juice).

Aim

Evaluate whether a shortened dose and time of treatment as well as the pharmaceutical presentation in rosemary tea (Rosmarinus officinalis) instead of powder have a therapeutic effect in the treatment of T2D.

Method

The complementary therapy with Rosemary tea (2g/1 litre of water per day) were evaluate on resistance to insulin, oxidative stress, biochemical parameters and anthropometric measurements in forty patients T2D under treatment with metformin and/or glibenclamide afther giving your authorization through informed consent.

Producción Científica de la Universidad Autónoma de Zacatecas UAZ

Article

BIOLOGÍA Y QUÍMICA Rosemary resistance to insulin anthropometric parameters